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  • Detection of FGFR2:FAM76A Fusion Gene in Circulating Tumor RNA Based on Catalytic Signal Amplification of Graphene Oxide-loaded Magnetic Nanoparticles

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    Accepted Manuscript (AM)
    Author(s)
    Gorgannezhad, Lena
    Umer, Muhammad
    Masud, Mostafa Kamal
    Hossain, Md Shahriar A
    Tanaka, S
    Yamauchi, Y
    Salomon, Carlos
    Kline, Richard
    Nguyen, Nam-Trung
    Shiddiky, Muhammad JA
    Griffith University Author(s)
    Nguyen, Nam-Trung
    Shiddiky, Muhammad J.
    Umer, Muhammad
    Year published
    2018
    Metadata
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    Abstract
    Circulating tumor nucleic acids (ctNAs) are promising biomarkers for minimally invasive cancer assessment. The FGFR2 : FAM76A fusion gene is one of the highly promising ovarian cancer biomarkers detectable in ctNAs. Herein, we introduce a new amplification‐free electrochemical assay for the detection of FGFR2 : FAM76A fusion gene in ctNAs extracted from ovarian cancer patients. The assay relies on the electrocatalytic activity of a new class of superparamagnetic graphene‐loaded iron oxide nanoparticles (GO‐NPFe2O3). After isolation and purification, the target RNA was directly adsorbed onto the GO‐NPFe2O3 surface through ...
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    Circulating tumor nucleic acids (ctNAs) are promising biomarkers for minimally invasive cancer assessment. The FGFR2 : FAM76A fusion gene is one of the highly promising ovarian cancer biomarkers detectable in ctNAs. Herein, we introduce a new amplification‐free electrochemical assay for the detection of FGFR2 : FAM76A fusion gene in ctNAs extracted from ovarian cancer patients. The assay relies on the electrocatalytic activity of a new class of superparamagnetic graphene‐loaded iron oxide nanoparticles (GO‐NPFe2O3). After isolation and purification, the target RNA was directly adsorbed onto the GO‐NPFe2O3 surface through graphene‐RNA affinity interaction. The electrocatalytic signal was achieved by the reduction of surface‐attached ruthenium hexaammine(III) chloride which was further amplified by using the ferricyanide redox system. Our assay depicted an excellent detection sensitivity down to 1.0 fM, high specificity and excellent reproducibility (% RSD=<5 %, for n=3). The analytical performance of our method was validated with standard qRT‐PCR analysis. We believe that this newly developed assay would be practically applicable in clinical research.
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    Journal Title
    Electroanalysis
    Volume
    30
    Issue
    10
    DOI
    https://doi.org/10.1002/elan.201800282
    Copyright Statement
    © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. This is the peer reviewed version of the following article: 'Detection of FGFR2 : FAM76A Fusion Gene in Circulating Tumor RNA Based on Catalytic Signal Amplification of Graphene Oxide‐loaded Magnetic Nanoparticles', Electroanalysis, Volume 30, Issue 10, Pages 2293-2301, October 2018, which has been published in final form at https://doi.org/10.1002/elan.201800282. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving (http://olabout.wiley.com/WileyCDA/Section/id-828039.html)
    Subject
    Analytical chemistry
    Physical chemistry
    Other chemical sciences
    Medical biotechnology diagnostics (incl. biosensors)
    Publication URI
    http://hdl.handle.net/10072/381312
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    • Journal articles

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