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  • The Role of Chikungunya nsP3 in Regulating G3BP Activity, Stress Granule Formation and Drug Efficacy

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    Lu,Xue_Final Thesis_Redacted.pdf (2.934Mb)
    Author(s)
    Lu, Xue
    Primary Supervisor
    Kennedy, Hendrick
    Other Supervisors
    Watters, Dianne
    Year published
    2018-06
    Metadata
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    Abstract
    Ras-GTPase activating protein SH3 domain binding proteins (G3BP) are a small family of RNA-binding proteins that have been implicated in regulating gene expression when a cell undergoes transformation or in response to external stimuli. Changes in the cellular expression of G3BPs are correlated to several cancers including head and neck, thyroid, colon, pancreatic and breast cancer. G3BPs are components of stress granules (SGs) and can regulate their formation. SGs are specialised ribonucleoprotein (RNP) particles that respond to cellular stresses, including chemotherapy, to triage mRNA with selected transcripts being degraded, ...
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    Ras-GTPase activating protein SH3 domain binding proteins (G3BP) are a small family of RNA-binding proteins that have been implicated in regulating gene expression when a cell undergoes transformation or in response to external stimuli. Changes in the cellular expression of G3BPs are correlated to several cancers including head and neck, thyroid, colon, pancreatic and breast cancer. G3BPs are components of stress granules (SGs) and can regulate their formation. SGs are specialised ribonucleoprotein (RNP) particles that respond to cellular stresses, including chemotherapy, to triage mRNA with selected transcripts being degraded, stored or translated. The net result to the cell is a change in the gene expression which confers a survival response to the cell. This can include the upregulation of genes needed to survive external stresses and this can include the development of drug resistance in cells. Many viruses, including Chikungunya, target SG and dismantle the granules so that the cell cannot respond to the viral infection. Non-structural protein3 (nsP3), from the Chikungunya virus, has been shown to translocate G3BP from SG, and the inability of the cell to form SG is required for the virus to complete an infectious cycle. Interestingly, the formation of SG is correlated to drug-resistance and blocking their formation has been shown to reestablish the efficacy of the chemotherapeutic, bortezomib. The present study explored the disruption of SG formation, caused by the interaction of nsP3 with G3BP, and as a result restored cytotoxicity of bortezomib and improved the selectivity of the bortezomib for cancer cell lines when compared to HEK cells. In addition, the interaction of nsP3 with G3BP also disrupted the activity of G3BP and led to the significantly downregulation of G3BP target genes NDUFV1 and IFITM3. These findings represent a tool for future study into strategies to block G3BP activities and disrupt SG formation in anti-cancer therapeutics.
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    Thesis Type
    Thesis (Masters)
    Degree Program
    Master of Science (MSc)
    School
    School of Environment and Sc
    DOI
    https://doi.org/10.25904/1912/3604
    Copyright Statement
    The author owns the copyright in this thesis, unless stated otherwise.
    Subject
    Chikungunya
    nsP3
    G3BP activity
    Stress
    Granule formation
    Drug efficacy
    Publication URI
    http://hdl.handle.net/10072/381505
    Collection
    • Theses - Higher Degree by Research

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