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dc.contributor.advisorKennedy, Hendrick
dc.contributor.authorLu, Xue
dc.date.accessioned2018-12-12T03:33:22Z
dc.date.available2018-12-12T03:33:22Z
dc.date.issued2018-06
dc.identifier.urihttp://hdl.handle.net/10072/381505
dc.description.abstractRas-GTPase activating protein SH3 domain binding proteins (G3BP) are a small family of RNA-binding proteins that have been implicated in regulating gene expression when a cell undergoes transformation or in response to external stimuli. Changes in the cellular expression of G3BPs are correlated to several cancers including head and neck, thyroid, colon, pancreatic and breast cancer. G3BPs are components of stress granules (SGs) and can regulate their formation. SGs are specialised ribonucleoprotein (RNP) particles that respond to cellular stresses, including chemotherapy, to triage mRNA with selected transcripts being degraded, stored or translated. The net result to the cell is a change in the gene expression which confers a survival response to the cell. This can include the upregulation of genes needed to survive external stresses and this can include the development of drug resistance in cells. Many viruses, including Chikungunya, target SG and dismantle the granules so that the cell cannot respond to the viral infection. Non-structural protein3 (nsP3), from the Chikungunya virus, has been shown to translocate G3BP from SG, and the inability of the cell to form SG is required for the virus to complete an infectious cycle. Interestingly, the formation of SG is correlated to drug-resistance and blocking their formation has been shown to reestablish the efficacy of the chemotherapeutic, bortezomib. The present study explored the disruption of SG formation, caused by the interaction of nsP3 with G3BP, and as a result restored cytotoxicity of bortezomib and improved the selectivity of the bortezomib for cancer cell lines when compared to HEK cells. In addition, the interaction of nsP3 with G3BP also disrupted the activity of G3BP and led to the significantly downregulation of G3BP target genes NDUFV1 and IFITM3. These findings represent a tool for future study into strategies to block G3BP activities and disrupt SG formation in anti-cancer therapeutics.en_US
dc.languageEnglish
dc.language.isoen
dc.publisherGriffith University
dc.publisher.placeBrisbane
dc.subject.keywordsChikungunyaen_US
dc.subject.keywordsnsP3en_US
dc.subject.keywordsG3BP activityen_US
dc.subject.keywordsStressen_US
dc.subject.keywordsGranule formationen_US
dc.subject.keywordsDrug efficacyen_US
dc.titleThe Role of Chikungunya nsP3 in Regulating G3BP Activity, Stress Granule Formation and Drug Efficacyen_US
dc.typeGriffith thesisen_US
gro.facultyScience, Environment, Engineering and Technologyen_US
gro.rights.copyrightThe author owns the copyright in this thesis, unless stated otherwise.
gro.hasfulltextFull Text
dc.contributor.otheradvisorWatters, Dianne
gro.thesis.degreelevelThesis (Masters)en_US
gro.thesis.degreeprogramMaster of Science (MSc)en_US
gro.departmentSchool of Environment and Scen_US


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