Laccase Immobilization onto Magnetic β-Cyclodextrin-Modified Chitosan: Improved Enzyme Stability and Efficient Performance for Phenolic Compounds Elimination
Author(s)
Tarasi, Roghayeh
Alipour, Masoumeh
Gorgannezhad, Lena
Imanparast, Somaye
Yousefi-Ahmadipour, Aliakbar
Ramezani, Ali
Ganjali, Mohammad Reza
Shafiee, Abbas
Faramarzi, Mohammad Ali
Khoobi, Mehdi
Griffith University Author(s)
Year published
2018
Metadata
Show full item recordAbstract
Three types of improved Fe3O4 magnetic nanoparticles (MNPs), including poly(amidoisophthalicacid) coated magnetite nanoparticles (Fe@PA), cyclodextrin (CD) anchored Fe@PA (Fe@PA-CD), and chitosan (Cs) coated Fe@PA-CD (Fe@PACD-Cs) were successfully developed and characterized. Laccase immobilization onto MNPs was carried out via physical adsorption. The maximal and minimal loading capacity were obtained for Fe@PA and Fe@PA-CD-Cs, respectively. Fe@PA-CDCs-laccase exhibited around 100% of the maximum activity at pH 4 and maintained 70% of its initial activity within the temperature range of 15–55 °C; and Cs coated nanoparticles ...
View more >Three types of improved Fe3O4 magnetic nanoparticles (MNPs), including poly(amidoisophthalicacid) coated magnetite nanoparticles (Fe@PA), cyclodextrin (CD) anchored Fe@PA (Fe@PA-CD), and chitosan (Cs) coated Fe@PA-CD (Fe@PACD-Cs) were successfully developed and characterized. Laccase immobilization onto MNPs was carried out via physical adsorption. The maximal and minimal loading capacity were obtained for Fe@PA and Fe@PA-CD-Cs, respectively. Fe@PA-CDCs-laccase exhibited around 100% of the maximum activity at pH 4 and maintained 70% of its initial activity within the temperature range of 15–55 °C; and Cs coated nanoparticles were more efficient than non-coated. Fe@PA-CD-Cs-laccase maintained 70% of its initial activity up to 12 d from the first day of storage at 25 °C whereas the free laccase, Fe@PA-laccase, and Fe@PA-CD-laccase kept 10%, 28%, and 33% of initial activity, respectively. Furthermore, bio-removal of phenolic compounds was performed by the free and immobilized enzyme. Fe@PA-CD-Cs-laccase showed maximal removal with 96.4% and 85.5% for phenol and bisphenol A, respectively. It seems that Fe@PA-CD-Cs could be an appropriate support for immobilization of other enzymes in various industrial application especially bioremoval of phenolic compounds. Open image in new window
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View more >Three types of improved Fe3O4 magnetic nanoparticles (MNPs), including poly(amidoisophthalicacid) coated magnetite nanoparticles (Fe@PA), cyclodextrin (CD) anchored Fe@PA (Fe@PA-CD), and chitosan (Cs) coated Fe@PA-CD (Fe@PACD-Cs) were successfully developed and characterized. Laccase immobilization onto MNPs was carried out via physical adsorption. The maximal and minimal loading capacity were obtained for Fe@PA and Fe@PA-CD-Cs, respectively. Fe@PA-CDCs-laccase exhibited around 100% of the maximum activity at pH 4 and maintained 70% of its initial activity within the temperature range of 15–55 °C; and Cs coated nanoparticles were more efficient than non-coated. Fe@PA-CD-Cs-laccase maintained 70% of its initial activity up to 12 d from the first day of storage at 25 °C whereas the free laccase, Fe@PA-laccase, and Fe@PA-CD-laccase kept 10%, 28%, and 33% of initial activity, respectively. Furthermore, bio-removal of phenolic compounds was performed by the free and immobilized enzyme. Fe@PA-CD-Cs-laccase showed maximal removal with 96.4% and 85.5% for phenol and bisphenol A, respectively. It seems that Fe@PA-CD-Cs could be an appropriate support for immobilization of other enzymes in various industrial application especially bioremoval of phenolic compounds. Open image in new window
View less >
Journal Title
Macromolecular Research
Volume
26
Issue
8
Subject
Macromolecular and materials chemistry
Macromolecular and materials chemistry not elsewhere classified
Magnetic nanoparticles
Chitosan
Laccase
Immobilization
Enzyme stability