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  • Laccase Immobilization onto Magnetic β-Cyclodextrin-Modified Chitosan: Improved Enzyme Stability and Efficient Performance for Phenolic Compounds Elimination

    Author(s)
    Tarasi, Roghayeh
    Alipour, Masoumeh
    Gorgannezhad, Lena
    Imanparast, Somaye
    Yousefi-Ahmadipour, Aliakbar
    Ramezani, Ali
    Ganjali, Mohammad Reza
    Shafiee, Abbas
    Faramarzi, Mohammad Ali
    Khoobi, Mehdi
    Griffith University Author(s)
    Gorgannezhad, Lena
    Year published
    2018
    Metadata
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    Abstract
    Three types of improved Fe3O4 magnetic nanoparticles (MNPs), including poly(amidoisophthalicacid) coated magnetite nanoparticles (Fe@PA), cyclodextrin (CD) anchored Fe@PA (Fe@PA-CD), and chitosan (Cs) coated Fe@PA-CD (Fe@PACD-Cs) were successfully developed and characterized. Laccase immobilization onto MNPs was carried out via physical adsorption. The maximal and minimal loading capacity were obtained for Fe@PA and Fe@PA-CD-Cs, respectively. Fe@PA-CDCs-laccase exhibited around 100% of the maximum activity at pH 4 and maintained 70% of its initial activity within the temperature range of 15–55 °C; and Cs coated nanoparticles ...
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    Three types of improved Fe3O4 magnetic nanoparticles (MNPs), including poly(amidoisophthalicacid) coated magnetite nanoparticles (Fe@PA), cyclodextrin (CD) anchored Fe@PA (Fe@PA-CD), and chitosan (Cs) coated Fe@PA-CD (Fe@PACD-Cs) were successfully developed and characterized. Laccase immobilization onto MNPs was carried out via physical adsorption. The maximal and minimal loading capacity were obtained for Fe@PA and Fe@PA-CD-Cs, respectively. Fe@PA-CDCs-laccase exhibited around 100% of the maximum activity at pH 4 and maintained 70% of its initial activity within the temperature range of 15–55 °C; and Cs coated nanoparticles were more efficient than non-coated. Fe@PA-CD-Cs-laccase maintained 70% of its initial activity up to 12 d from the first day of storage at 25 °C whereas the free laccase, Fe@PA-laccase, and Fe@PA-CD-laccase kept 10%, 28%, and 33% of initial activity, respectively. Furthermore, bio-removal of phenolic compounds was performed by the free and immobilized enzyme. Fe@PA-CD-Cs-laccase showed maximal removal with 96.4% and 85.5% for phenol and bisphenol A, respectively. It seems that Fe@PA-CD-Cs could be an appropriate support for immobilization of other enzymes in various industrial application especially bioremoval of phenolic compounds. Open image in new window
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    Journal Title
    Macromolecular Research
    Volume
    26
    Issue
    8
    DOI
    https://doi.org/10.1007/s13233-018-6095-z
    Subject
    Macromolecular and materials chemistry
    Macromolecular and materials chemistry not elsewhere classified
    Magnetic nanoparticles
    Chitosan
    Laccase
    Immobilization
    Enzyme stability
    Publication URI
    http://hdl.handle.net/10072/381850
    Collection
    • Journal articles

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