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dc.contributor.authorMayr, Juliane
dc.contributor.authorLau, Kam
dc.contributor.authorLai, Jimmy CC
dc.contributor.authorGagarinov, Ivan A
dc.contributor.authorShi, Yun
dc.contributor.authorMcAtamney, Sarah
dc.contributor.authorChan, Renee WY
dc.contributor.authorNicholls, John
dc.contributor.authorvon Itzstein, Mark
dc.contributor.authorHaselhorst, Thomas
dc.date.accessioned2019-05-29T13:16:07Z
dc.date.available2019-05-29T13:16:07Z
dc.date.issued2018
dc.identifier.issn2045-2322
dc.identifier.doi10.1038/s41598-018-34175-3
dc.identifier.urihttp://hdl.handle.net/10072/382119
dc.description.abstractThe initial stage of host cell infection by influenza A viruses (IAV) is mediated through interaction of the viral haemagglutinin (HA) with cell surface glycans. The binding requirement of IAVs for Galβ(1,4) Glc/ GlcNAc (lactose/lactosamine) glycans with a terminal α(2,6)-linked (human receptors) or α(2,3)- linked (avian receptors) N-acetylneuraminic residue commonly found on N-glycans, is well-established. However the role and significance of sialylated Galβ(1,3)GalNAc (core 1) epitopes that are typical Oglycoforms in influenza virus pathogenesis remains poorly detailed. Here we report a multidisciplinary study using NMR spectroscopy, virus neutralization assays and molecular modelling, into the potential for IAV to engage sialyl-Galβ(1,3)GalNAc O-glycoforms for cell attachment. H5 containing virus like particles (VLPs) derived from an H5N1 avian IAV strain show a significant involvement of the O-glycanspecific GalNAc residue, coordinated by a EQTKLY motif conserved in highly pathogenic avian influenza (HPAI) strains. Notably, human pandemic H1N1 influenza viruses shift the preference from ‘humanlike’ α(2,6)-linkages in sialylated Galβ(1,4)Glc/GlcNAc fragments to ‘avian-like’ α(2,3)-linkages in sialylated Galβ(1,3)GalNAc without involvement of the GalNAc residue. Overall, our study suggests that sialylated Galβ(1,3)GalNAc as O-glycan core 1 glycoforms are involved in the influenza A virus life cycle and play a particularly crucial role during infection of HPAI strains.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherNature Publishing Group
dc.publisher.placeUnited Kingdom
dc.relation.ispartofchapter16382
dc.relation.ispartofpagefrom1
dc.relation.ispartofpageto12
dc.relation.ispartofjournalScientific Reports
dc.relation.ispartofvolume8
dc.subject.fieldofresearchOther biomedical and clinical sciences not elsewhere classified
dc.subject.fieldofresearchOther health sciences not elsewhere classified
dc.subject.fieldofresearchcode329999
dc.subject.fieldofresearchcode429999
dc.titleUnravelling the Role of O-glycans in Influenza A Virus Infection
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttp://creativecommons.org/licenses/by/4.0/
dc.description.versionVersion of Record (VoR)
gro.facultyOffice of the Snr Dep Vice Chancellor, Institute for Glycomics
gro.rights.copyright© The Author(s) 2018. This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 International (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
gro.hasfulltextFull Text
gro.griffith.authorvon Itzstein, Mark
gro.griffith.authorHaselhorst, Thomas E.
gro.griffith.authorShi, Yun


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