Isomeric Separation and Characterisation of Glycoconjugates
Author(s)
Alagesan, Kathirvel
Everest-Dass, Arun
Kolarich, Daniel
Year published
2018
Metadata
Show full item recordAbstract
Individual monosaccharides can be linked in a variety of different combinations to form complex glycoconjugates. In contrast to DNA and proteins, glycoconjugate synthesis does not follow any template but is the consequence of the concerted action of various enzymes such as transferases and glycosidases. Thus, tools for glycoconjugate sequencing need to differentiate individual monosaccharide identity, linkage and anomericity to investigate and understand glycoconjugate function. In this chapter we provide a concise overview on the most commonly used and robust tools to separate and characterise glycoconjugate isomers.Individual monosaccharides can be linked in a variety of different combinations to form complex glycoconjugates. In contrast to DNA and proteins, glycoconjugate synthesis does not follow any template but is the consequence of the concerted action of various enzymes such as transferases and glycosidases. Thus, tools for glycoconjugate sequencing need to differentiate individual monosaccharide identity, linkage and anomericity to investigate and understand glycoconjugate function. In this chapter we provide a concise overview on the most commonly used and robust tools to separate and characterise glycoconjugate isomers.
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Journal Title
Advances in Experimental Medicine and Biology
Volume
1104
Subject
Biomedical and clinical sciences
Glycomics
Glycoproteomics
N-glycan
O-glycan
Porous graphitized carbon
PGC
HILIC
HPLC