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  • Isomeric Separation and Characterisation of Glycoconjugates

    Author(s)
    Alagesan, Kathirvel
    Everest-Dass, Arun
    Kolarich, Daniel
    Griffith University Author(s)
    Kolarich, Daniel
    Everest-Dass, Arun
    Alagesan, Kathirvel
    Year published
    2018
    Metadata
    Show full item record
    Abstract
    Individual monosaccharides can be linked in a variety of different combinations to form complex glycoconjugates. In contrast to DNA and proteins, glycoconjugate synthesis does not follow any template but is the consequence of the concerted action of various enzymes such as transferases and glycosidases. Thus, tools for glycoconjugate sequencing need to differentiate individual monosaccharide identity, linkage and anomericity to investigate and understand glycoconjugate function. In this chapter we provide a concise overview on the most commonly used and robust tools to separate and characterise glycoconjugate isomers.Individual monosaccharides can be linked in a variety of different combinations to form complex glycoconjugates. In contrast to DNA and proteins, glycoconjugate synthesis does not follow any template but is the consequence of the concerted action of various enzymes such as transferases and glycosidases. Thus, tools for glycoconjugate sequencing need to differentiate individual monosaccharide identity, linkage and anomericity to investigate and understand glycoconjugate function. In this chapter we provide a concise overview on the most commonly used and robust tools to separate and characterise glycoconjugate isomers.
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    Journal Title
    Advances in Experimental Medicine and Biology
    Volume
    1104
    DOI
    https://doi.org/10.1007/978-981-13-2158-0_5
    Subject
    Biomedical and clinical sciences
    Glycomics
    Glycoproteomics
    N-glycan
    O-glycan
    Porous graphitized carbon
    PGC
    HILIC
    HPLC
    Publication URI
    http://hdl.handle.net/10072/382150
    Collection
    • Journal articles

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