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dc.contributor.authorEscher, Beate I
dc.contributor.authorNeale, Peta A
dc.contributor.authorVilleneuve, Daniel L
dc.date.accessioned2019-07-04T12:41:38Z
dc.date.available2019-07-04T12:41:38Z
dc.date.issued2018
dc.identifier.issn0730-7268
dc.identifier.doi10.1002/etc.4178
dc.identifier.urihttp://hdl.handle.net/10072/382169
dc.description.abstractIn vitro assays and high‐throughput screening (HTS) tools are increasingly being employed as replacements for animal testing, but most concentration–response curves are still evaluated with models developed for animal testing. We argue that application of in vitro assays, particularly reporter gene assays, to environmental samples can benefit from a different approach to concentration–response modeling. First, cytotoxicity often occurs at higher concentrations, especially for weakly acting compounds and in complex environmental mixtures with many components. In these cases, specific effects can be masked by cytotoxicity. Second, for many HTS assays, low effect levels can be precisely quantified because of the low variability of controls in cell‐based assays and the opportunity to run many concentrations and replicates when using high‐density well‐plate formats (e.g., 384 or more wells per plate). Hence, we recommend focusing concentration–response modeling on the lower portion of the concentration–response curve, which is approximately linear. Effect concentrations derived from low–effect level linear concentration–response models facilitate simple derivation of relative effect potencies and the correct application of mixture toxicity models in the calculation of bioanalytical equivalent concentrations. Environ Toxicol Chem 2018;37:2273–2280. © 2018 SETAC
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherJohn Wiley & Sons
dc.publisher.placeUnited States
dc.relation.ispartofpagefrom2273
dc.relation.ispartofpageto2280
dc.relation.ispartofissue9
dc.relation.ispartofjournalEnvironmental Toxicology and Chemistry
dc.relation.ispartofvolume37
dc.relation.urihttp://purl.org/au-research/grants/NHMRC/APP1074775
dc.relation.grantIDAPP1074775
dc.relation.fundersNHMRC
dc.subject.fieldofresearchEnvironmental Sciences not elsewhere classified
dc.subject.fieldofresearchChemical Sciences
dc.subject.fieldofresearchEnvironmental Sciences
dc.subject.fieldofresearchBiological Sciences
dc.subject.fieldofresearchcode059999
dc.subject.fieldofresearchcode03
dc.subject.fieldofresearchcode05
dc.subject.fieldofresearchcode06
dc.subject.keywordsBioanalytical equivalent concentration
dc.subject.keywordsIn vitro toxicology
dc.subject.keywordsEnvironmental toxicology
dc.subject.keywordsDose–response modeling
dc.titleThe advantages of linear concentration-response curves for in vitro bioassays with environmental samples
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.rights.copyright© 2018 SETAC. This is the peer reviewed version of the following article: The Advantages of Linear Concentration–Response Curves for In Vitro Bioassays with Environmental Samples, Environmental Toxicology and Chemistry, Volume 37, Number 9, pp. 2273–2280, 2018, which has been published in final form at 10.1002/etc.4178. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving (http://olabout.wiley.com/WileyCDA/Section/id-828039.html)
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gro.griffith.authorNeale, Peta A.
gro.griffith.authorEscher, Beate


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