• myGriffith
    • Staff portal
    • Contact Us⌄
      • Future student enquiries 1800 677 728
      • Current student enquiries 1800 154 055
      • International enquiries +61 7 3735 6425
      • General enquiries 07 3735 7111
      • Online enquiries
      • Staff phonebook
    View Item 
    •   Home
    • Griffith Research Online
    • Journal articles
    • View Item
    • Home
    • Griffith Research Online
    • Journal articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

  • All of Griffith Research Online
    • Communities & Collections
    • Authors
    • By Issue Date
    • Titles
  • This Collection
    • Authors
    • By Issue Date
    • Titles
  • Statistics

  • Most Popular Items
  • Statistics by Country
  • Most Popular Authors
  • Support

  • Contact us
  • FAQs
  • Admin login

  • Login
  • Type III secretion inhibitors for the management of bacterial plant diseases

    View/Open
    Davis90824-Accepted.pdf (410.4Kb)
    File version
    Accepted Manuscript (AM)
    Author(s)
    Puigvert, Marina
    Sole, Montserrat
    Lopez-Garcia, Belen
    Coll, Nuria S
    Beattie, Karren D
    Davis, Rohan A
    Elofsson, Mikael
    Valls, Marc
    Griffith University Author(s)
    Davis, Rohan A.
    Mitchell, Karren D.
    Year published
    2019
    Metadata
    Show full item record
    Abstract
    The identification of chemical compounds that prevent and combat bacterial diseases is fundamental for crop production. Bacterial virulence inhibitors are a promising alternative to classical control treatments, because they have a low environmental impact and are less likely to generate bacterial resistance. The major virulence determinant of most animal and plant bacterial pathogens is the type III secretion system (T3SS). In this work, we screened nine plant extracts and 12 isolated compounds—including molecules effective against human pathogens—for their capacity to inhibit the T3SS of plant pathogens and for their ...
    View more >
    The identification of chemical compounds that prevent and combat bacterial diseases is fundamental for crop production. Bacterial virulence inhibitors are a promising alternative to classical control treatments, because they have a low environmental impact and are less likely to generate bacterial resistance. The major virulence determinant of most animal and plant bacterial pathogens is the type III secretion system (T3SS). In this work, we screened nine plant extracts and 12 isolated compounds—including molecules effective against human pathogens—for their capacity to inhibit the T3SS of plant pathogens and for their applicability as virulence inhibitors for crop protection. The screen was performed using a luminescent reporter system developed in the model pathogenic bacterium Ralstonia solanacearum. Five synthetic molecules, one natural product and two plant extracts were found to down‐regulate T3SS transcription, most through the inhibition of the regulator hrpB. In addition, for three of the molecules, corresponding to salicylidene acylhydrazide derivatives, the inhibitory effect caused a dramatic decrease in the secretion capacity, which was translated into impaired plant responses. These candidate virulence inhibitors were then tested for their ability to protect plants. We demonstrated that salicylidene acylhydrazides can limit R. solanacearum multiplication in planta and protect tomato plants from bacterial speck caused by Pseudomonas syringae pv. tomato. Our work validates the efficiency of transcription reporters to discover compounds or natural product extracts that can be potentially applied to prevent bacterial plant diseases.
    View less >
    Journal Title
    Molecular Plant Pathology
    Volume
    20
    Issue
    1
    DOI
    https://doi.org/10.1111/mpp.12736
    Copyright Statement
    © 2019 BSPP and John Wiley & Sons Ltd. This is the peer reviewed version of the following article: Type III secretion inhibitors for the management of bacterial plant diseases, Molecular Plant Pathology, 20 (1), pp. 20-32, 2019, which has been published in final form at https://doi.org/10.1111/mpp.12736. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving (http://olabout.wiley.com/WileyCDA/Section/id-828039.html)
    Subject
    Crop and pasture production
    Microbiology
    Plant biology
    Plant biology not elsewhere classified
    Virulence inhibitors
    Type III secretion system
    Ralstonia solanacearum
    Pseudomonas syringae
    Disease control
    Bacterial wilt
    Bacterial speck
    Publication URI
    http://hdl.handle.net/10072/382390
    Collection
    • Journal articles

    Footer

    Disclaimer

    • Privacy policy
    • Copyright matters
    • CRICOS Provider - 00233E

    Tagline

    • Gold Coast
    • Logan
    • Brisbane - Queensland, Australia
    First Peoples of Australia
    • Aboriginal
    • Torres Strait Islander