Megahertz serial crystallography
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Author(s)
Wiedorn, Max O
Oberthuer, Dominik
Bean, Richard
Schubert, Robin
Werner, Nadine
Abbey, Brian
Aepfelbacher, Martin
Adriano, Luigi
Allahgholi, Aschkan
Al-Qudami, Nasser
Andreasson, Jakob
Aplin, Steve
Awel, Salah
Ayyer, Kartik
Bajt, Sasa
Barak, Imrich
Bari, Sadia
Bielecki, Johan
Botha, Sabine
Boukhelef, Djelloul
Brehm, Wolfgang
Brockhauser, Sandor
Cheviakov, Igor
Coleman, Matthew A
Cruz-Mazo, Francisco
Danilevski, Cyril
Darmanin, Connie
Doak, R Bruce
Domaracky, Martin
Doerner, Katerina
Du, Yang
Fangohr, Hans
Fleckenstein, Holger
Frank, Matthias
Fromme, Petra
Ganan-Calvo, Alfonso M
Gevorkov, Yaroslav
Giewekemeyer, Klaus
Ginn, Helen Mary
Graafsma, Heinz
Graceffa, Rita
Greiffenberg, Dominic
Gumprecht, Lars
Goettlicher, Peter
Hajdu, Janos
Hauf, Steffen
Heymann, Michael
Holmes, Susannah
Horke, Daniel A
Hunter, Mark S
Imlau, Siegfried
Kaukher, Alexander
Kim, Yoonhee
Klyuev, Alexander
Knoska, Juraj
Kobe, Bostjan
Kuhn, Manuela
Kupitz, Christopher
Kuepper, Jochen
Lahey-Rudolph, Janine Mia
Laurus, Torsten
Le Cong, Karoline
Letrun, Romain
Xavier, P Lourdu
Maia, Luis
Maia, Filipe RNC
Mariani, Valerio
Messerschmidt, Marc
Metz, Markus
Mezza, Davide
Michelat, Thomas
Mills, Grant
Monteiro, Diana CF
Morgan, Andrew
Muhlig, Kerstin
Munke, Anna
Muennich, Astrid
Nette, Julia
Nugent, Keith A
Nuguid, Theresa
Orville, Allen M
Pandey, Suraj
Pena, Gisel
Villanueva-Perez, Pablo
Poehlsen, Jennifer
Previtali, Gianpietro
Redecke, Lars
Riekehr, Winnie Maria
Rohde, Holger
Round, Adam
Safenreiter, Tatiana
Sarrou, Iosifina
Sato, Tokushi
Schmidt, Marius
Schmitt, Bernd
Schoenherr, Robert
Schulz, Joachim
Sellberg, Jonas A
Seibert, M Marvin
Seuring, Carolin
Shelby, Megan L
Shoeman, Robert L
Sikorski, Marcin
Silenzi, Alessandro
Stan, Claudiu A
Shi, Xintian
Stern, Stephan
Sztuk-Dambietz, Jola
Szuba, Janusz
Tolstikova, Aleksandra
Trebbin, Martin
Trunk, Ulrich
Vagovic, Patrik
Ve, Thomas
Weinhausen, Britta
White, Thomas A
Wrona, Krzysztof
Xu, Chen
Yefanov, Oleksandr
Zatsepin, Nadia
Zhang, Jiaguo
Perbandt, Markus
Mancuso, Adrian P
Betzel, Christian
Chapman, Henry
Barty, Anton
Griffith University Author(s)
Year published
2018
Metadata
Show full item recordAbstract
The new European X-ray Free-Electron Laser is the first X-ray free-electron laser capable of delivering X-ray pulses with a megahertz inter-pulse spacing, more than four orders of magnitude higher than previously possible. However, to date, it has been unclear whether it would indeed be possible to measure high-quality diffraction data at megahertz pulse repetition rates. Here, we show that high-quality structures can indeed be obtained using currently available operating conditions at the European XFEL. We present two complete data sets, one from the well-known model system lysozyme and the other from a so far unknown complex ...
View more >The new European X-ray Free-Electron Laser is the first X-ray free-electron laser capable of delivering X-ray pulses with a megahertz inter-pulse spacing, more than four orders of magnitude higher than previously possible. However, to date, it has been unclear whether it would indeed be possible to measure high-quality diffraction data at megahertz pulse repetition rates. Here, we show that high-quality structures can indeed be obtained using currently available operating conditions at the European XFEL. We present two complete data sets, one from the well-known model system lysozyme and the other from a so far unknown complex of a β-lactamase from K. pneumoniae involved in antibiotic resistance. This result opens up megahertz serial femtosecond crystallography (SFX) as a tool for reliable structure determination, substrate screening and the efficient measurement of the evolution and dynamics of molecular structures using megahertz repetition rate pulses available at this new class of X-ray laser source.
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View more >The new European X-ray Free-Electron Laser is the first X-ray free-electron laser capable of delivering X-ray pulses with a megahertz inter-pulse spacing, more than four orders of magnitude higher than previously possible. However, to date, it has been unclear whether it would indeed be possible to measure high-quality diffraction data at megahertz pulse repetition rates. Here, we show that high-quality structures can indeed be obtained using currently available operating conditions at the European XFEL. We present two complete data sets, one from the well-known model system lysozyme and the other from a so far unknown complex of a β-lactamase from K. pneumoniae involved in antibiotic resistance. This result opens up megahertz serial femtosecond crystallography (SFX) as a tool for reliable structure determination, substrate screening and the efficient measurement of the evolution and dynamics of molecular structures using megahertz repetition rate pulses available at this new class of X-ray laser source.
View less >
Journal Title
Nature Communications
Volume
9
Copyright Statement
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing,
adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/ licenses/by/4.0/.
© The Author(s) 2018 NATURE COMMUNICATIONS | (2018) 9:4025 | DOI: 10.1038/s41467-018-06156-7 | www.nature.com/naturecommunications 9
Subject
Molecular medicine
Megahertz pulse
Crystallography
Serial femtosecond crystallography (SFX)