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  • Complementary Imaging of Silver Nanoparticle Interactions with Green Algae: Dark-Field Microscopy, Electron Microscopy, and Nanoscale Secondary Ion Mass Spectrometry

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    Author(s)
    Sekine, Ryo
    Moore, Katie L
    Matzke, Marianne
    Vallotton, Pascal
    Jiang, Haibo
    Hughes, Gareth M
    Kirby, Jason K
    Donner, Erica
    Grovenor, Chris RM
    Svendsen, Claus
    Lombi, Enzo
    Griffith University Author(s)
    Sekine, Ryo
    Year published
    2017
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    Abstract
    Increasing consumer use of engineered nanomaterials has led to significantly increased efforts to understand their potential impact on the environment and living organisms. Currently, no individual technique can provide all the necessary information such as their size, distribution, and chemistry in complex biological systems. Consequently, there is a need to develop complementary instrumental imaging approaches that provide enhanced understanding of these “bio-nano” interactions to overcome the limitations of individual techniques. Here we used a multimodal imaging approach incorporating dark-field light microscopy, ...
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    Increasing consumer use of engineered nanomaterials has led to significantly increased efforts to understand their potential impact on the environment and living organisms. Currently, no individual technique can provide all the necessary information such as their size, distribution, and chemistry in complex biological systems. Consequently, there is a need to develop complementary instrumental imaging approaches that provide enhanced understanding of these “bio-nano” interactions to overcome the limitations of individual techniques. Here we used a multimodal imaging approach incorporating dark-field light microscopy, high-resolution electron microscopy, and nanoscale secondary ion mass spectrometry (NanoSIMS). The aim was to gain insight into the bio-nano interactions of surface-functionalized silver nanoparticles (Ag-NPs) with the green algae Raphidocelis subcapitata, by combining the fidelity, spatial resolution, and elemental identification offered by the three techniques, respectively. Each technique revealed that Ag-NPs interact with the green algae with a dependence on the size (10 nm vs 60 nm) and surface functionality (tannic acid vs branched polyethylenimine, bPEI) of the NPs. Dark-field light microscopy revealed the presence of strong light scatterers on the algal cell surface, and SEM imaging confirmed their nanoparticulate nature and localization at nanoscale resolution. NanoSIMS imaging confirmed their chemical identity as Ag, with the majority of signal concentrated at the cell surface. Furthermore, SEM and NanoSIMS provided evidence of 10 nm bPEI Ag-NP internalization at higher concentrations (40 μg/L), correlating with the highest toxicity observed from these NPs. This multimodal approach thus demonstrated an effective approach to complement dose–response studies in nano-(eco)-toxicological investigations.
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    Journal Title
    ACS Nano
    Volume
    11
    Issue
    11
    DOI
    https://doi.org/10.1021/acsnano.7b04556
    Copyright Statement
    This is an open access article published under a Creative Commons Attribution (CC-BY) License, which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.
    Subject
    Other environmental sciences not elsewhere classified
    Bio-nano interactions
    Dark-field light microscopy
    Multimodal imaging
    NanoSIMS
    Nanotoxicology
    Silver nanoparticles
    Publication URI
    http://hdl.handle.net/10072/382419
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