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dc.contributor.authorSekine, Ryo
dc.contributor.authorMoore, Katie L
dc.contributor.authorMatzke, Marianne
dc.contributor.authorVallotton, Pascal
dc.contributor.authorJiang, Haibo
dc.contributor.authorHughes, Gareth M
dc.contributor.authorKirby, Jason K
dc.contributor.authorDonner, Erica
dc.contributor.authorGrovenor, Chris RM
dc.contributor.authorSvendsen, Claus
dc.contributor.authorLombi, Enzo
dc.date.accessioned2019-02-20T04:46:17Z
dc.date.available2019-02-20T04:46:17Z
dc.date.issued2017
dc.identifier.issn1936-0851
dc.identifier.doi10.1021/acsnano.7b04556
dc.identifier.urihttp://hdl.handle.net/10072/382419
dc.description.abstractIncreasing consumer use of engineered nanomaterials has led to significantly increased efforts to understand their potential impact on the environment and living organisms. Currently, no individual technique can provide all the necessary information such as their size, distribution, and chemistry in complex biological systems. Consequently, there is a need to develop complementary instrumental imaging approaches that provide enhanced understanding of these “bio-nano” interactions to overcome the limitations of individual techniques. Here we used a multimodal imaging approach incorporating dark-field light microscopy, high-resolution electron microscopy, and nanoscale secondary ion mass spectrometry (NanoSIMS). The aim was to gain insight into the bio-nano interactions of surface-functionalized silver nanoparticles (Ag-NPs) with the green algae Raphidocelis subcapitata, by combining the fidelity, spatial resolution, and elemental identification offered by the three techniques, respectively. Each technique revealed that Ag-NPs interact with the green algae with a dependence on the size (10 nm vs 60 nm) and surface functionality (tannic acid vs branched polyethylenimine, bPEI) of the NPs. Dark-field light microscopy revealed the presence of strong light scatterers on the algal cell surface, and SEM imaging confirmed their nanoparticulate nature and localization at nanoscale resolution. NanoSIMS imaging confirmed their chemical identity as Ag, with the majority of signal concentrated at the cell surface. Furthermore, SEM and NanoSIMS provided evidence of 10 nm bPEI Ag-NP internalization at higher concentrations (40 μg/L), correlating with the highest toxicity observed from these NPs. This multimodal approach thus demonstrated an effective approach to complement dose–response studies in nano-(eco)-toxicological investigations.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherAmerican Chemical Society
dc.relation.ispartofpagefrom10894
dc.relation.ispartofpageto10902
dc.relation.ispartofissue11
dc.relation.ispartofjournalACS Nano
dc.relation.ispartofvolume11
dc.subject.fieldofresearchEnvironmental Sciences not elsewhere classified
dc.subject.fieldofresearchcode059999
dc.subject.keywordsBio-nano interactions
dc.subject.keywordsDark-field light microscopy
dc.subject.keywordsMultimodal imaging
dc.subject.keywordsNanoSIMS
dc.subject.keywordsNanotoxicology
dc.subject.keywordsSilver nanoparticles
dc.titleComplementary Imaging of Silver Nanoparticle Interactions with Green Algae: Dark-Field Microscopy, Electron Microscopy, and Nanoscale Secondary Ion Mass Spectrometry
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dc.description.versionVersion of Record (VoR)
gro.rights.copyrightThis is an open access article published under a Creative Commons Attribution (CC-BY) License, which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.
gro.hasfulltextFull Text
gro.griffith.authorSekine, Ryo


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