Epigenetically reprogrammed methylation landscape drives the DNA self-assembly and serves as a universal cancer biomarker
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Author(s)
Ibn Sina, Abu Ali
Carrascosa, Laura G
Liang, Ziyu
Grewal, Yadveer S
Wardiana, Andri
Shiddiky, Muhammad JA
Gardiner, Robert A
Samaratunga, Hemamali
Gandhi, Maher K
Scott, Rodney J
Korbie, Darren
Trau, Matt
Griffith University Author(s)
Year published
2018
Metadata
Show full item recordAbstract
Epigenetic reprogramming in cancer genomes creates a distinct methylation landscape
encompassing clustered methylation at regulatory regions separated by large intergenic
tracks of hypomethylated regions. This methylation landscape that we referred to as
Methylscape is displayed by most cancer types, thus may serve as a universal cancer biomarker.
To-date most research has focused on the biological consequences of DNA
Methylscape changes whereas its impact on DNA physicochemical properties remains
unexplored. Herein, we examine the effect of levels and genomic distribution of methylcytosines
on the physicochemical properties ...
View more >Epigenetic reprogramming in cancer genomes creates a distinct methylation landscape encompassing clustered methylation at regulatory regions separated by large intergenic tracks of hypomethylated regions. This methylation landscape that we referred to as Methylscape is displayed by most cancer types, thus may serve as a universal cancer biomarker. To-date most research has focused on the biological consequences of DNA Methylscape changes whereas its impact on DNA physicochemical properties remains unexplored. Herein, we examine the effect of levels and genomic distribution of methylcytosines on the physicochemical properties of DNA to detect the Methylscape biomarker. We find that DNA polymeric behaviour is strongly affected by differential patterning of methylcytosine, leading to fundamental differences in DNA solvation and DNA-gold affinity between cancerous and normal genomes. We exploit these Methylscape differences to develop simple, highly sensitive and selective electrochemical or colorimetric one-step assays for the detection of cancer. These assays are quick, i.e., analysis time ≤10 minutes, and require minimal sample preparation and small DNA input.
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View more >Epigenetic reprogramming in cancer genomes creates a distinct methylation landscape encompassing clustered methylation at regulatory regions separated by large intergenic tracks of hypomethylated regions. This methylation landscape that we referred to as Methylscape is displayed by most cancer types, thus may serve as a universal cancer biomarker. To-date most research has focused on the biological consequences of DNA Methylscape changes whereas its impact on DNA physicochemical properties remains unexplored. Herein, we examine the effect of levels and genomic distribution of methylcytosines on the physicochemical properties of DNA to detect the Methylscape biomarker. We find that DNA polymeric behaviour is strongly affected by differential patterning of methylcytosine, leading to fundamental differences in DNA solvation and DNA-gold affinity between cancerous and normal genomes. We exploit these Methylscape differences to develop simple, highly sensitive and selective electrochemical or colorimetric one-step assays for the detection of cancer. These assays are quick, i.e., analysis time ≤10 minutes, and require minimal sample preparation and small DNA input.
View less >
Journal Title
Nature Communications
Volume
9
Issue
1
Copyright Statement
© The Author(s) 2018 This article is licensed under a Creative Commons
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Subject
Oncology and carcinogenesis not elsewhere classified
Epigenetic reprogramming
Cancer genomes
Methylscape
DNA polymeric behaviour