Graphene-Oxide-Loaded Superparamagnetic Iron Oxide Nanoparticles for Ultrasensitive Electrocatalytic Detection of MicroRNA
Author(s)
Islam, Nazmul
Gorgannezhad, Lena
Masud, Mostafa Kamal
Tanaka, Shunsuke
Hossain, Md. Shahriar A.
Yamauchi, Yusuke
Nguyen, Nam-Trung
Shiddiky, Muhammad
Year published
2018
Metadata
Show full item recordAbstract
We report the electrocatalytic activity of a new class of
superparamagnetic nanoparticles, graphene-oxide-loaded iron
oxide (GO/IO hybrid material), towards the reduction of
ruthenium hexaammine(III) chloride (Ru(NH3)6]3+, RuHex). Leveraging
the electrocatalytic activity of the GO/IO hybrid material
and the signal enhancement capacity of [Ru(NH3)6]3+/[Fe(CN)6]3
in an electrocatalytic cycle, an ultrasensitive and specific electrochemical
sensor was developed for the detection of cancerrelated
microRNA (miRNA). Using the direct affinity interaction
between RNA and graphene oxide, magnetically isolated and purified target miRNA ...
View more >We report the electrocatalytic activity of a new class of superparamagnetic nanoparticles, graphene-oxide-loaded iron oxide (GO/IO hybrid material), towards the reduction of ruthenium hexaammine(III) chloride (Ru(NH3)6]3+, RuHex). Leveraging the electrocatalytic activity of the GO/IO hybrid material and the signal enhancement capacity of [Ru(NH3)6]3+/[Fe(CN)6]3 in an electrocatalytic cycle, an ultrasensitive and specific electrochemical sensor was developed for the detection of cancerrelated microRNA (miRNA). Using the direct affinity interaction between RNA and graphene oxide, magnetically isolated and purified target miRNA were directly adsorbed onto a screenprinted electrode modified with the GO/IO hybrid material. The detection was enabled by chronocoulometric (CC) readout of charge-compensating [Ru(NH3)6]3+ followed by an enhancement in CC charge display through the Ru(NH3)6]3+ /[Fe(CN)6]3 system. We demonstrate an excellent limit of detection of 1.0 fM by accurately detecting miR-21 in synthetic samples and showcase its clinical utility in ovarian cancer cell lines with high sensitivity (ten cells) and good reproducibility (%RSD= <5%, for n=3).
View less >
View more >We report the electrocatalytic activity of a new class of superparamagnetic nanoparticles, graphene-oxide-loaded iron oxide (GO/IO hybrid material), towards the reduction of ruthenium hexaammine(III) chloride (Ru(NH3)6]3+, RuHex). Leveraging the electrocatalytic activity of the GO/IO hybrid material and the signal enhancement capacity of [Ru(NH3)6]3+/[Fe(CN)6]3 in an electrocatalytic cycle, an ultrasensitive and specific electrochemical sensor was developed for the detection of cancerrelated microRNA (miRNA). Using the direct affinity interaction between RNA and graphene oxide, magnetically isolated and purified target miRNA were directly adsorbed onto a screenprinted electrode modified with the GO/IO hybrid material. The detection was enabled by chronocoulometric (CC) readout of charge-compensating [Ru(NH3)6]3+ followed by an enhancement in CC charge display through the Ru(NH3)6]3+ /[Fe(CN)6]3 system. We demonstrate an excellent limit of detection of 1.0 fM by accurately detecting miR-21 in synthetic samples and showcase its clinical utility in ovarian cancer cell lines with high sensitivity (ten cells) and good reproducibility (%RSD= <5%, for n=3).
View less >
Journal Title
ChemElectroChem
Volume
5
Issue
17
Copyright Statement
© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. This is the peer reviewed version of the following article: Graphene‐Oxide‐Loaded Superparamagnetic Iron Oxide Nanoparticles for Ultrasensitive Electrocatalytic Detection of MicroRNA, ChemElectroChem, Vol. 5, 2488–2495, 2018, which has been published in final form at 10.1002/celc.201800339. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving (http://olabout.wiley.com/WileyCDA/Section/id-828039.html)
Subject
Analytical chemistry
Physical chemistry
Other chemical sciences
Other chemical sciences not elsewhere classified
Macromolecular and materials chemistry
Organic chemistry
Electrocatalytic activity
Superparamagnetic nanoparticles
Iron oxide
MicroRNA