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  • Proteomic analysis of Plasmodium falciparum histone deacetylase 1 complex proteins

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    Engel158585.pdf (3.593Mb)
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    post-print
    Author(s)
    Engel, Jessica A
    Norris, Emma L
    Gilson, Paul
    Przyborski, Jude
    Shonhai, Addmore
    Blatch, Gregory L
    Skinner-Adams, Tina S
    Gorman, Jeffrey
    Headlam, Madeleine
    Andrews, Katherine T
    Griffith University Author(s)
    Skinner-Adams, Tina
    Andrews, Katherine T.
    Year published
    2019
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    Abstract
    Plasmodium falciparum histone deacetylases (PfHDACs) are an important class of epigenetic regulators that alter protein lysine acetylation, contributing to regulation of gene expression and normal parasite growth and development. PfHDACs are therefore under investigation as drug targets for malaria. Despite this, our understanding of the biological roles of these enzymes is only just beginning to emerge. In higher eukaryotes, HDACs function as part of multi-protein complexes and act on both histone and non-histone substrates. Here, we present a proteomics analysis of PfHDAC1 immunoprecipitates, identifying 26 putative P. ...
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    Plasmodium falciparum histone deacetylases (PfHDACs) are an important class of epigenetic regulators that alter protein lysine acetylation, contributing to regulation of gene expression and normal parasite growth and development. PfHDACs are therefore under investigation as drug targets for malaria. Despite this, our understanding of the biological roles of these enzymes is only just beginning to emerge. In higher eukaryotes, HDACs function as part of multi-protein complexes and act on both histone and non-histone substrates. Here, we present a proteomics analysis of PfHDAC1 immunoprecipitates, identifying 26 putative P. falciparum complex proteins in trophozoite-stage asexual intraerythrocytic parasites. The co-migration of two of these (P. falciparum heat shock proteins 70-1 and 90) with PfHDAC1 was validated using Blue Native PAGE combined with Western blot. These data provide a snapshot of possible PfHDAC1 interactions and a starting point for future studies focused on elucidating the broader function of PfHDACs in Plasmodium parasites.
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    Journal Title
    Experimental Parasitology
    Volume
    198
    DOI
    https://doi.org/10.1016/j.exppara.2019.01.008
    Copyright Statement
    © 2019 Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International Licence (http://creativecommons.org/licenses/by-nc-nd/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, providing that the work is properly cited.
    Subject
    Microbiology
    Veterinary sciences
    Publication URI
    http://hdl.handle.net/10072/382915
    Collection
    • Journal articles

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