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dc.contributor.authorYang, Tao
dc.contributor.authorZhang, Bing
dc.contributor.authorPat, Betty K
dc.contributor.authorWei, Ming Q
dc.contributor.authorGobe, Glenda C
dc.date.accessioned2017-05-03T13:11:39Z
dc.date.available2017-05-03T13:11:39Z
dc.date.issued2010
dc.date.modified2011-05-06T06:48:52Z
dc.identifier.issn1110-7243
dc.identifier.doi10.1155/2010/859240
dc.identifier.urihttp://hdl.handle.net/10072/38294
dc.description.abstractBackground. Lentiviral constructs reportedly can integrate into the genome of non-dividing, terminally differentiated cells and dividing cells, for long-term gene expression. This investigation tested whether a third generation lentiviral-mediated small interfering RNA (siRNA) delivered into renal epithelial and fibroblast cells against type II transforming growth factor-beta receptor (siRNA-TBRII) could better attenuate renal fibrogenesis in comparison with a non-lentiviral construct. Methods. HIV-derived lentiviral and non-lentiviral constructs were used to transfect cells with siRNA-TBRII or siRNA-EGFP control. Human embryonic kidney (HEK-293T), renal epithelial cells (NRK-52E) and renal fibroblasts (NRK-49F) were transfected and gene silencing quantified (fluorescence microscopy, Western blotting, fluorescence-activated cell sorting). Renal fibrogenesis was assessed using extracellular matrix protein synthesis (fibronectin and collagen-III; Western immunoblot), and a-smooth muscle actin (a- SMA) was analysed as a marker of fibroblast activation and epithelial-to-mesenchymal transdifferentiation (EMT). Results. Lentiviral-mediated siRNA-TBRII significantly suppressed TBRII expression in all cell lines, and also significantly suppressed renal fibrogenesis. In comparison with the non-lentiviral construct, lentiviral-mediated siRNA-TBRII produced stronger and more persistent inhibition of collagen-III in NRK-49F cells, fibronectin in all renal cell lines, and a-SMA in renal epithelial cells. Conclusions. Lentiviral vector systems against TBRII can be delivered into renal cells to efficiently limit renal fibrogenesis by sequence-specific gene silencing.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.format.extent4623316 bytes
dc.format.mimetypeapplication/pdf
dc.languageEnglish
dc.language.isoeng
dc.publisherHindawi Publishing Corporation
dc.publisher.placeUnited States
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom859240-1
dc.relation.ispartofpageto859240-12
dc.relation.ispartofjournalJournal of Biomedicine and Biotechnology
dc.relation.ispartofvolume2010
dc.rights.retentionY
dc.subject.fieldofresearchBiological sciences
dc.subject.fieldofresearchInformation and computing sciences
dc.subject.fieldofresearchMedical biochemistry and metabolomics not elsewhere classified
dc.subject.fieldofresearchcode31
dc.subject.fieldofresearchcode46
dc.subject.fieldofresearchcode320599
dc.titleLentiviral-Mediated RNA Interference against TGF-Beta Receptor Type II in Renal Epithelial and Fibroblast Cell Populations In Vitro Demonstrates Regulated Renal Fibrogenesis That Is More Efficient than a Nonlentiviral Vector
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttp://creativecommons.org/licenses/by-nc-sa/2.1/au/
gro.rights.copyright© The Author(s) 2010. The attached file is posted here with permission of the copyright owners for your personal use only. No further distribution permitted.For information about this journal please refer to the journal's website. The online version of this work is licensed under a Creative Commons License, available at http://creativecommons.org/licenses/by-nc-sa/2.1/au/
gro.date.issued2010
gro.hasfulltextFull Text
gro.griffith.authorWei, Ming Q.


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