dc.contributor.author | Clarke, Daniel | |
dc.contributor.author | Idris, Adi | |
dc.contributor.author | McMillan, Nigel AJ | |
dc.date.accessioned | 2019-05-29T12:47:25Z | |
dc.date.available | 2019-05-29T12:47:25Z | |
dc.date.issued | 2019 | |
dc.identifier.issn | 1932-6203 | |
dc.identifier.doi | 10.1371/journal.pone.0211954 | |
dc.identifier.uri | http://hdl.handle.net/10072/383016 | |
dc.description.abstract | Liposomes are versatile and well-proven as a means to deliver nucleic acids into cells. Most of the formulation procedures used are labour intensive and result in unstable end products. We have previously reported on the development of a simple, yet efficient, hydration-of-freeze-dried-matrix (HFDM) method to entrap siRNA within lipid particles. Here we show that the particles are stable up to 12 months after storage at room temperature (RT), 4°C or -20°C. While RT storage results in changes in particle size and polydispersity, gene silencing of all particles was similar to freshly prepared particles following storage for 3, 6, 9 or 12 months at all temperatures. This is the first report of such long-term stability in siRNA-loaded liposomes. | |
dc.description.peerreviewed | Yes | |
dc.language | English | |
dc.language.iso | eng | |
dc.publisher | PUBLIC LIBRARY SCIENCE | |
dc.relation.ispartofpagefrom | 1 | |
dc.relation.ispartofpageto | 8 | |
dc.relation.ispartofissue | 2 | |
dc.relation.ispartofjournal | PLOS ONE | |
dc.relation.ispartofvolume | 14 | |
dc.subject.fieldofresearch | Genetics | |
dc.subject.fieldofresearch | Pharmaceutical delivery technologies | |
dc.subject.fieldofresearchcode | 3105 | |
dc.subject.fieldofresearchcode | 321404 | |
dc.subject.keywords | Lipidic particles | |
dc.subject.keywords | siRNA delivery | |
dc.title | Development of novel lipidic particles for siRNA delivery that are highly effective after 12 months storage | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dc.type.code | C - Journal Articles | |
gro.rights.copyright | © 2019 Daniel Clarke et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |
gro.hasfulltext | Full Text | |
gro.griffith.author | McMillan, Nigel | |