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dc.contributor.authorClarke, Daniel
dc.contributor.authorIdris, Adi
dc.contributor.authorMcMillan, Nigel AJ
dc.date.accessioned2019-05-29T12:47:25Z
dc.date.available2019-05-29T12:47:25Z
dc.date.issued2019
dc.identifier.issn1932-6203
dc.identifier.doi10.1371/journal.pone.0211954
dc.identifier.urihttp://hdl.handle.net/10072/383016
dc.description.abstractLiposomes are versatile and well-proven as a means to deliver nucleic acids into cells. Most of the formulation procedures used are labour intensive and result in unstable end products. We have previously reported on the development of a simple, yet efficient, hydration-of-freeze-dried-matrix (HFDM) method to entrap siRNA within lipid particles. Here we show that the particles are stable up to 12 months after storage at room temperature (RT), 4°C or -20°C. While RT storage results in changes in particle size and polydispersity, gene silencing of all particles was similar to freshly prepared particles following storage for 3, 6, 9 or 12 months at all temperatures. This is the first report of such long-term stability in siRNA-loaded liposomes.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherPUBLIC LIBRARY SCIENCE
dc.relation.ispartofpagefrom1
dc.relation.ispartofpageto8
dc.relation.ispartofissue2
dc.relation.ispartofjournalPLOS ONE
dc.relation.ispartofvolume14
dc.subject.fieldofresearchGenetics
dc.subject.fieldofresearchPharmaceutical delivery technologies
dc.subject.fieldofresearchcode3105
dc.subject.fieldofresearchcode321404
dc.subject.keywordsLipidic particles
dc.subject.keywordssiRNA delivery
dc.titleDevelopment of novel lipidic particles for siRNA delivery that are highly effective after 12 months storage
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.rights.copyright© 2019 Daniel Clarke et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
gro.hasfulltextFull Text
gro.griffith.authorMcMillan, Nigel


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