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dc.contributor.authorOmar Lazaro-Castellanos, Jesus
dc.contributor.authorMata-Rosas, Martin
dc.contributor.authorGonzalez, Dolores
dc.contributor.authorArias, Salvador
dc.contributor.authorReverchon, Frederique
dc.date.accessioned2019-06-26T06:01:21Z
dc.date.available2019-06-26T06:01:21Z
dc.date.issued2018
dc.identifier.issn1054-5476
dc.identifier.doi10.1007/s11627-018-9908-z
dc.identifier.urihttp://hdl.handle.net/10072/383051
dc.description.abstractIn vitro propagation protocols were established for endangered species of cacti Mammillaria hernandezii, M. dixanthocentron, and M. lanata. In vitro-germinated seedlings were used as the explant source. Three explant types were evaluated as apical, basal, and lateral stem sections. Shoot multiplication was achieved using Murashige and Skoog (MS) medium supplemented with benzyladenine, kinetin, meta-topolin, and thidiazuron in equimolar concentrations (0.0, 0.4, 1.1, 2.2, 4.4, and 8.9 μM). Shoot regeneration was obtained primarily in the lateral stem section explants. In M. hernandezii, an average of 7.4 shoots was regenerated in MS medium with 2.2 μM meta-topolin. M. dixanthocentron and M. lanata averaged 16.7 and 17.9 shoots/explant, respectively, in MS medium supplemented with 1.1 μM meta-topolin. Rooting occurred in MS medium without growth regulators. Three in vitro culture cycles were performed to validate the propagation protocols and to verify genetic stability. Shoots were collected in each cycle and genomic DNA was extracted. Amplified microsatellites were used to compare each genotype with its respective donor plant. Polymorphic information content analysis showed low levels of intra-clonal polymorphisms—M. hernandezii 0.04 and M. dixanthocentron and M. lanata both 0.12. More than 95% of the plants were successfully acclimatized in the greenhouse. After 12 months, plants of M. hernandezii reached the flowering stage; M. dixanthocentron and M. lanata flowered at 24 mo.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherSPRINGER
dc.relation.ispartofpagefrom518
dc.relation.ispartofpageto529
dc.relation.ispartofissue5
dc.relation.ispartofjournalIN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
dc.relation.ispartofvolume54
dc.subject.fieldofresearchPlant biology
dc.subject.fieldofresearchcode3108
dc.titleIn vitro propagation of endangered Mammillaria genus (Cactaceae) species and genetic stability assessment using SSR markers
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.hasfulltextNo Full Text
gro.griffith.authorReverchon, Frederique


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