A rapid, maskless 3D prototyping for fabrication of capillary circuits: Toward urinary protein detection

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Author(s)
Yan, Sheng
Zhu, Yuanqing
Tang, Shi-Yang
Li, Yuxing
Zhao, Qianbin
Yuan, Dan
Yun, Guolin
Zhang, Jun
Zhang, Shiwu
Li, Weihua
Griffith University Author(s)
Year published
2018
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Show full item recordAbstract
Proteinuria is an established risk marker for progressive renal function loss and patients would significantly benefit from a point‐of‐care testing. Although extensive work has been done to develop the microfluidic devices for the detection of urinary protein, they need the complicated operation and bulky peripherals. Here, we present a rapid, maskless 3D prototyping for fabrication of capillary fluidic circuits using laser engraving. The capillary circuits can be fabricated in a short amount of time (<10 min) without the requirements of clean‐room facilities and photomasks. The advanced capillary components (e.g., trigger ...
View more >Proteinuria is an established risk marker for progressive renal function loss and patients would significantly benefit from a point‐of‐care testing. Although extensive work has been done to develop the microfluidic devices for the detection of urinary protein, they need the complicated operation and bulky peripherals. Here, we present a rapid, maskless 3D prototyping for fabrication of capillary fluidic circuits using laser engraving. The capillary circuits can be fabricated in a short amount of time (<10 min) without the requirements of clean‐room facilities and photomasks. The advanced capillary components (e.g., trigger valves, retention valves and retention bursting valves) were fabricated, enabling the sequential liquid delivery and sample‐reagent mixing. With the integration of smartphone‐based detection platform, the microfluidic device can quantify the urinary protein via a colorimetric analysis. By eliminating the bulky and expensive equipment, this smartphone‐based detection platform is portable for on‐site quantitative detection.
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View more >Proteinuria is an established risk marker for progressive renal function loss and patients would significantly benefit from a point‐of‐care testing. Although extensive work has been done to develop the microfluidic devices for the detection of urinary protein, they need the complicated operation and bulky peripherals. Here, we present a rapid, maskless 3D prototyping for fabrication of capillary fluidic circuits using laser engraving. The capillary circuits can be fabricated in a short amount of time (<10 min) without the requirements of clean‐room facilities and photomasks. The advanced capillary components (e.g., trigger valves, retention valves and retention bursting valves) were fabricated, enabling the sequential liquid delivery and sample‐reagent mixing. With the integration of smartphone‐based detection platform, the microfluidic device can quantify the urinary protein via a colorimetric analysis. By eliminating the bulky and expensive equipment, this smartphone‐based detection platform is portable for on‐site quantitative detection.
View less >
Journal Title
ELECTROPHORESIS
Volume
39
Issue
7
Copyright Statement
© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. This is the peer reviewed version of the following article: A rapid, maskless 3D prototyping for fabrication of capillary circuits: Toward urinary protein detection, Electrophoresis, Volume39, Issue7,
April 2018, which has been published in final form at https://doi.org/10.1002/elps.201700449. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving (http://olabout.wiley.com/WileyCDA/Section/id-828039.html)
Subject
Analytical chemistry
Biochemistry and cell biology
Chemical engineering