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dc.contributor.authorBartnikowski, Michal
dc.contributor.authorMoon, Ho-Jin
dc.contributor.authorIvanovski, Saso
dc.date.accessioned2019-06-08T01:32:19Z
dc.date.available2019-06-08T01:32:19Z
dc.date.issued2018
dc.identifier.issn1748-6041
dc.identifier.doi10.1088/1748-605X/aad916
dc.identifier.urihttp://hdl.handle.net/10072/383178
dc.description.abstractThe immunomodulatory effects of lithium have been reported across a range of models and contexts. Lithium appears to have a positive effect on osteogenesis in vivo, while in vitro outcomes throughout the literature are varied. Tissue engineering approaches have rarely targeted local lithium delivery within a regenerative setting. We hypothesized that part of the positive effects of lithium in vivo may be due to an immunomodulatory effect manifesting in a local environment. To achieve a sustained lithium release from scaffold constructs, we blended lithium carbonate, a soluble salt of lithium, with the biomaterial polymer polycaprolactone (PCL). We printed constructs of PCL alone, and with 5% (5Li) and 10% (10Li) lithium carbonate. Mechanical testing revealed that mechanical properties were largely retained with lithium carbonate incorporation, and we measured a consistent release of the ion over a 7 day period. The efficacy of our construct system was then assessed using a primary mouse macrophage culture, and a differentiated osteoclast culture. We found that the lithium released from constructs had a great effect on macrophage polarization, resulting in pronounced upregulation of immunomodulatory (M2) genes, and a decrease in pro-inflammatory (M1) genes. This was reflected in cytokine expression, and illustrated through immunofluorescent staining. Osteoclast activity was greatly suppressed by the lithium incorporation, with a marked effect on gene expression and actin ring formation. Our work demonstrated an effective system for local lithium delivery, confirmed the pronounced effects that lithium has on macrophage and osteoclast response, and sets the stage for further innovations in ion release for targeted tissue engineering.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherIOP PUBLISHING LTD
dc.relation.ispartofissue6
dc.relation.ispartofjournalBIOMEDICAL MATERIALS
dc.relation.ispartofvolume13
dc.subject.fieldofresearchBiomedical engineering
dc.subject.fieldofresearchMedical biotechnology
dc.subject.fieldofresearchcode4003
dc.subject.fieldofresearchcode3206
dc.titleRelease of lithium from 3D printed polycaprolactone scaffolds regulates macrophage and osteoclast response
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.hasfulltextNo Full Text
gro.griffith.authorMoon, Ho-Jin


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