Evaluating the mechanisms of the impacts of key factors on soil soluble organic nitrogen concentrations in subtropical mountain ecosystems

Abstract

Soil soluble organic nitrogen (SON) concentrations in terrestrial ecosystems were influenced differently and substantially by both biotic and abiotic factors. This study aimed to ascertain the mechanisms of the impact of the key factors on the SON concentrations of subtropical mountain ecosystems in southeastern China using an integrative approach, which combined a field plot survey, gray relational analysis and structure equation modeling. The results showed that the soil organic matter, clay content, protease activity and bacterial biomass were the key factors controlling the dynamics of the SON concentrations in subtropical mountain ecosystems. Protease activity, by catalyzing the degradation of complex organic nitrogen to SON, had the highest direct influence on the SON concentrations among all of the impact factors with direct impact effect of 0.44. Organic matter, which serves as a primary source of SON and can increase soil protease activity and bacterial biomass, contributed the most significantly to the SON concentrations in both direct and indirect pathways with total impact effects of 0.87. Clay, by adsorbing SON and affecting organic matter accumulation and protease activity, also had important direct or indirect influences on the SON concentrations with total impact effects of 0.48. The impact of the bacterial biomass on the SON concentrations was likely to be concealed by accompanying nitrogen-degrading enzyme activity with total impact effects of 0.22. Thus, the organic matter, clay content and protease activity exerted greater total impact effects on the SON concentrations compared with the bacterial biomass. Protease activity and organic matter had a greater positive direct impact on the SON concentrations compared with the bacterial biomass and clay content, while organic matter also had greater positive indirect impacts on the SON concentrations than did the clay content. This study's results could help to elucidate the differential mechanism of SON dynamics among various terrestrial ecosystems.