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dc.contributor.authorAlowaidi, Faisal
dc.contributor.authorHashimi, Saeed M
dc.contributor.authorNguyen, Maria
dc.contributor.authorMeshram, Mallika
dc.contributor.authorAlqurashi, Naif
dc.contributor.authorCavanagh, Brenton L
dc.contributor.authorBellette, Bernadette
dc.contributor.authorIvanovski, Saso
dc.contributor.authorMeedenyia, Adrian
dc.contributor.authorWood, Stephen A
dc.description.abstractCripto‐1 has been implicated in a number of human cancers. Although there is high potential for a role of Cripto‐1 in glioblastoma multiforme (GBM) pathogenesis and progression, few studies have tried to define its role in GBM. These studies were limited in that Cripto‐1 expression was not studied in detail in relation to markers of cancer initiation and progression. Therefore, these correlative studies allowed limited interpretation of Criptos‐1's effect on the various aspects of GBM development using the U87 GBM cell line. In this study, we sought to delineate the role of Cripto‐1 in facilitating pathogenesis, stemness, proliferation, invasion, migration and angiogenesis in GBM. Our findings show that upon overexpressing Cripto‐1 in U87 GBM cells, the stemness markers Nanog, Oct4, Sox2, and CD44 increased expression. Similarly, an increase in Ki67 was observed demonstrating Cripto‐1's potential to induce cellular proliferation. Likewise, we report a novel finding that increased expression of the markers of migration and invasion, Vimentin and Twist, correlated with upregulation of Cripto‐1. Moreover, Cripto‐1 exposure led to VEGFR‐2 overexpression along with higher tube formation under conditions promoting endothelial growth. Taken together our results support a role for Cripto‐1 in the initiation, development, progression, and maintenance of GBM pathogenesis. The data presented here are also consistent with a role for Cripto‐1 in the re‐growth and invasive growth in GBM. This highlights its potential use as a predictive and diagnostic marker in GBM as well as a therapeutic target.
dc.relation.ispartofjournalJOURNAL OF CELLULAR BIOCHEMISTRY
dc.subject.fieldofresearchBiochemistry and cell biology
dc.subject.fieldofresearchMedical physiology
dc.subject.keywordsEpithelial to mesenchymal transition (EMT)
dc.titleInvestigating the role of CRIPTO-1 (TDGF-1) in glioblastoma multiforme U87 cell line
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.hasfulltextNo Full Text
gro.griffith.authorHashimi, Saeed M.
gro.griffith.authorAlqurashi, Naif
gro.griffith.authorIvanovski, Saso
gro.griffith.authorWood, Stephen A.
gro.griffith.authorAlowaidi, Faisal
gro.griffith.authorMeshram, Mallika
gro.griffith.authorCavanagh, Brenton L.
gro.griffith.authorBellette, Bernadette
gro.griffith.authorMeedeniya, Adrian C.
gro.griffith.authorNguyen, Maria T.

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