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dc.contributor.authorAlowaidi, Faisal
dc.contributor.authorHashimi, Saeed Mujahid
dc.contributor.authorAlqurashi, Naif
dc.contributor.authorAlhulais, Reem
dc.contributor.authorIvanovski, Saso
dc.contributor.authorBellette, Bernadette
dc.contributor.authorMeedenyia, Adrian
dc.contributor.authorLam, Alfred
dc.contributor.authorWood, Stephen
dc.date.accessioned2019-07-04T12:35:04Z
dc.date.available2019-07-04T12:35:04Z
dc.date.issued2018
dc.identifier.issn1021-335X
dc.identifier.doi10.3892/or.2018.6392
dc.identifier.urihttp://hdl.handle.net/10072/383243
dc.description.abstractTo date two questions that remain unanswered regarding cancer are the following: i) how is it initiated, and ii) what is the role that cancer stem cells (CSCs) play in the disease process? Understanding the biology of CSCs and how they are generated is pivotal for the development of successful treatment regimens. To date, the lack of a representative cell model has prevented the successful identification and eradication of CSCs in vivo. The current methods of CSC identification are dependent on the protocol used to generate these cells, which has introduced variation and made the identification process more complicated. Furthermore, the list of possible markers is increasing in complexity. This is further confounded by the fact that there is insufficient information to determine whether the cells these markers detect are truly self‑renewing stem cells or, instead, progenitor cells. In the present study, we investigated a novel cell line model, CSC480, which can be employed to assess CSC markers and for testing novel therapeutic regimens. CSC480 cells have been revealed to express markers of CSCs such as CD44, ALDH1 and Sox2, that have lower expression in the SW480 cell line. CSC480 cells also expressed higher levels of the cancer resistance marker, ABCG2 and had higher proliferative and growth capacity than SW480 cells. In the present study, we also evaluated a novel approach to identify different cell types present in heterogeneous cancer cell populations according to their proliferative ability using the proliferation marker 5‑ethynyl‑2'‑deoxyuridine (EdU). Furthermore, using EdU, we identified dormant cells with a modified label‑retaining cell (LRC) protocol. Through this novel LRC method, we assessed newly discovered markers of stemness to ascertain their capability to identify quiescent from dividing CSCs. In conclusion, the CSC480 cell line was an important model to be used in unravelling the underlying mechanisms that control fast‑dividing and partially self‑renewing stem cells (SCs) that may give rise to cancer.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherSPANDIDOS PUBL LTD
dc.relation.ispartofpagefrom2881
dc.relation.ispartofpageto2891
dc.relation.ispartofissue6
dc.relation.ispartofjournalONCOLOGY REPORTS
dc.relation.ispartofvolume39
dc.subject.fieldofresearchOncology and Carcinogenesis
dc.subject.fieldofresearchcode1112
dc.titleAssessing stemness and proliferation properties of the newly established colon cancer 'stem' cell line, CSC480 and novel approaches to identify dormant cancer cells
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.description.versionVersion of Record (VoR)
gro.rights.copyrightCopyright remains with authors 2018. This is an open access article distributed under the terms of Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) License (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits unrestricted, non-commercial use, distribution and reproduction in any medium, providing that the work is properly cited.
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gro.griffith.authorHashimi, Saeed M.
gro.griffith.authorAlqurashi, Naif
gro.griffith.authorIvanovski, Saso
gro.griffith.authorLam, Alfred K.
gro.griffith.authorWood, Stephen A.
gro.griffith.authorALHulais, Reem A.
gro.griffith.authorBellette, Bernadette
gro.griffith.authorMeedeniya, Adrian C.


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