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dc.contributor.authorIslam, Farhadul
dc.contributor.authorGopalan, Vinod
dc.contributor.authorLaw, Simon
dc.contributor.authorTang, Johnny Cheuk-on
dc.contributor.authorLam, Alfred King-yin
dc.date.accessioned2019-07-04T12:36:42Z
dc.date.available2019-07-04T12:36:42Z
dc.date.issued2019
dc.identifier.issn0046-8177
dc.identifier.doi10.1016/j.humpath.2018.11.033
dc.identifier.urihttp://hdl.handle.net/10072/383663
dc.description.abstractFamily with sequence similarity 134, member B (FAM134B) is an autophagy regulator of endoplasmic reticulum first discovered to be involved in the pathogenesis of esophageal squamous cell carcinoma (ESCC). The present study examined the functional behavior of FAM134B in cancer cells and the association of FAM134B expression with clinicopathologic factors in patients with ESCC. Expression at both the mRNA and protein levels was investigated using real-time polymerase chain reaction and immunohistochemistry. The results were correlated with the clinical and pathological features of the patients. In addition, in vitro functional assays were used to investigate the roles of FAM134B in ESCC cells in response to gene silencing with shRNA lentiviral particles. Overexpression of FAM134B mRNA and protein was present in 31.2% (n = 29/93) and 36.6% (n = 41/112), respectively, in tumors, whereas downregulation occurred in 39.8% (n = 37/93) and 63.4% (n = 71/112), respectively. Expression of FAM134B protein in ESCC correlated with histologic grade (P =.002) and pathologic stage (P =.012). In vitro suppression of FAM134B in ESCC induced significant reductions of cell proliferation and colony formation (P <.05). In addition, suppression of FAM134B caused reduction of wound healing, migration, and invasion capacities of ESCC. To conclude, FAM134B could play crucial roles in the initiation and progression of ESCC, and FAM134B protein expression has potential predictive value. Therefore, development of strategies targeting FAM134B could have therapeutic value in the management of patients with ESCC.
dc.description.peerreviewedYes
dc.languageEnglish
dc.publisherW B SAUNDERS CO-ELSEVIER INC
dc.relation.ispartofpagefrom1
dc.relation.ispartofpageto10
dc.relation.ispartofjournalHUMAN PATHOLOGY
dc.relation.ispartofvolume87
dc.subject.fieldofresearchClinical Sciences
dc.subject.fieldofresearchcode1103
dc.titleFAM134B promotes esophageal squamous cell carcinoma in vitro and its correlations with clinicopathologic features
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttp://creativecommons.org/licenses/by-nc-nd/4.0/
gro.rights.copyright© 2019 Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International Licence, which permits unrestricted, non-commercial use, distribution and reproduction in any medium, providing that the work is properly cited.
gro.hasfulltextFull Text
gro.griffith.authorGopalan, Vinod
gro.griffith.authorLam, Alfred K.
gro.griffith.authorIslam, Farhad


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