Investigation of whole cell meningococcal glycan interactions using high throughput glycobiology techniques: Glycan array and surface plasmon resonance
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Author(s)
Mubaiwa, TD
Hartley-Tassell, LE
Semchenko, EA
Day, CJ
Jennings, MP
Seib, KL
Year published
2019
Metadata
Show full item recordAbstract
A growing body of evidence suggests that glycans are important for meningococcal host-pathogen interactions and virulence. The development of glycobiology techniques such as glycan array analysis and surface plasmon resonance (SPR) has increased awareness of the importance of glycans in biological processes and has increased the interest of their study. While these techniques are more routinely used with purified proteins, there is growing interest in their applicability to cell-based studies, to better emulate host-pathogen interactions in vivo. Here we describe the use of glycan array analysis and SPR for the investigation ...
View more >A growing body of evidence suggests that glycans are important for meningococcal host-pathogen interactions and virulence. The development of glycobiology techniques such as glycan array analysis and surface plasmon resonance (SPR) has increased awareness of the importance of glycans in biological processes and has increased the interest of their study. While these techniques are more routinely used with purified proteins, there is growing interest in their applicability to cell-based studies, to better emulate host-pathogen interactions in vivo. Here we describe the use of glycan array analysis and SPR for the investigation of glycan binding by Neisseria meningitidis cells. Used together, these methods can help identify and characterize N. meningitidis glycointeractions.
View less >
View more >A growing body of evidence suggests that glycans are important for meningococcal host-pathogen interactions and virulence. The development of glycobiology techniques such as glycan array analysis and surface plasmon resonance (SPR) has increased awareness of the importance of glycans in biological processes and has increased the interest of their study. While these techniques are more routinely used with purified proteins, there is growing interest in their applicability to cell-based studies, to better emulate host-pathogen interactions in vivo. Here we describe the use of glycan array analysis and SPR for the investigation of glycan binding by Neisseria meningitidis cells. Used together, these methods can help identify and characterize N. meningitidis glycointeractions.
View less >
Book Title
Neisseria meningitidis: Methods and Protocols
Volume
1969
Funder(s)
NHMRC
Grant identifier(s)
APP1099278
APP1045235
Copyright Statement
© 2019 Springer. This is an electronic version of an article published in Methods in Molecular Biology, Vol. 1969:113-121, January 2019. Methods in Molecular Biology (Neisseria meningitidis) is available online at: http://link.springer.com/ with the open URL of your article.
Subject
Glycobiology
Other chemical sciences
Biochemistry and cell biology
Medicinal and biomolecular chemistry
Glycobiology
Glycan arrays
Surface plasmon resonance (SPR)