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dc.contributor.authorJen, FEC
dc.contributor.authorSeib, KL
dc.contributor.authorTan, A
dc.contributor.editorSeib, Kate L.
dc.contributor.editorPeak, Ian R.
dc.date.accessioned2019-06-10T01:33:16Z
dc.date.available2019-06-10T01:33:16Z
dc.date.issued2019
dc.identifier.issn1064-3745
dc.identifier.doi10.1007/978-1-4939-9202-7_6
dc.identifier.urihttp://hdl.handle.net/10072/384119
dc.description.abstractFragment analysis (or fragment length analysis) is a PCR-based method which allows quantification of the size and proportion of a DNA repeat tract length of a phase-variable region. Primers are labeled with a fluorescent dye, the resulting amplicons are processed by capillary electrophoresis, and results are analyzed for amplicon size and proportion by associated software (such as Peakscanner). Here we describe the process of designing primers and controls to screen for the number of repeats in a polymeric tract of a phase-variable gene in Neisseria meningitidis (the DNA methyltransferase ModA is used as an example, but this method can be applied to other phase-variable genes).
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherSpringer
dc.publisher.placeNew York, NY, Untied States
dc.relation.ispartofbooktitleNeisseria meningitidis: Methods and Protocols
dc.relation.ispartofpagefrom93
dc.relation.ispartofpageto104
dc.relation.ispartofseriesMethods in Molecular Biology
dc.relation.ispartofvolume1969
dc.relation.urihttp://purl.org/au-research/grants/NHMRC/APP1099279
dc.relation.urihttp://purl.org/au-research/grants/NHMRC/APP1045235
dc.relation.grantIDAPP1099279
dc.relation.grantIDAPP1045235
dc.relation.fundersNHMRC
dc.subject.fieldofresearchBiochemistry and cell biology
dc.subject.fieldofresearchOther chemical sciences
dc.subject.fieldofresearchMedicinal and biomolecular chemistry
dc.subject.fieldofresearchcode3101
dc.subject.fieldofresearchcode3499
dc.subject.fieldofresearchcode3404
dc.subject.keywordsFragment analysis
dc.subject.keywordsPolymeric DNA tract
dc.subject.keywordsPhase-variable gene
dc.subject.keywordsDNA repeat sequence
dc.titleScreening DNA repeat tracts of phase variable genes by fragment analysis
dc.typeBook chapter
dc.type.descriptionB1 - Chapters
dc.type.codeC - Journal Articles
gro.rights.copyright© 2019 Springer. This is an electronic version of an article published in Methods in Molecular Biology, Vol., 1969:93-104, 2019. Methods in Molecular Biology is available online at: http://link.springer.com/ with the open URL of your article.
gro.hasfulltextFull Text
gro.griffith.authorSeib, Kate
gro.griffith.authorJen, Freda E.


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