dc.contributor.author | Jen, FEC | |
dc.contributor.author | Seib, KL | |
dc.contributor.author | Tan, A | |
dc.contributor.editor | Seib, Kate L. | |
dc.contributor.editor | Peak, Ian R. | |
dc.date.accessioned | 2019-06-10T01:33:16Z | |
dc.date.available | 2019-06-10T01:33:16Z | |
dc.date.issued | 2019 | |
dc.identifier.issn | 1064-3745 | |
dc.identifier.doi | 10.1007/978-1-4939-9202-7_6 | |
dc.identifier.uri | http://hdl.handle.net/10072/384119 | |
dc.description.abstract | Fragment analysis (or fragment length analysis) is a PCR-based method which allows quantification of the size and proportion of a DNA repeat tract length of a phase-variable region. Primers are labeled with a fluorescent dye, the resulting amplicons are processed by capillary electrophoresis, and results are analyzed for amplicon size and proportion by associated software (such as Peakscanner). Here we describe the process of designing primers and controls to screen for the number of repeats in a polymeric tract of a phase-variable gene in Neisseria meningitidis (the DNA methyltransferase ModA is used as an example, but this method can be applied to other phase-variable genes). | |
dc.description.peerreviewed | Yes | |
dc.language | English | |
dc.language.iso | eng | |
dc.publisher | Springer | |
dc.publisher.place | New York, NY, Untied States | |
dc.relation.ispartofbooktitle | Neisseria meningitidis: Methods and Protocols | |
dc.relation.ispartofpagefrom | 93 | |
dc.relation.ispartofpageto | 104 | |
dc.relation.ispartofseries | Methods in Molecular Biology | |
dc.relation.ispartofvolume | 1969 | |
dc.relation.uri | http://purl.org/au-research/grants/NHMRC/APP1099279 | |
dc.relation.uri | http://purl.org/au-research/grants/NHMRC/APP1045235 | |
dc.relation.grantID | APP1099279 | |
dc.relation.grantID | APP1045235 | |
dc.relation.funders | NHMRC | |
dc.subject.fieldofresearch | Biochemistry and cell biology | |
dc.subject.fieldofresearch | Other chemical sciences | |
dc.subject.fieldofresearch | Medicinal and biomolecular chemistry | |
dc.subject.fieldofresearchcode | 3101 | |
dc.subject.fieldofresearchcode | 3499 | |
dc.subject.fieldofresearchcode | 3404 | |
dc.subject.keywords | Fragment analysis | |
dc.subject.keywords | Polymeric DNA tract | |
dc.subject.keywords | Phase-variable gene | |
dc.subject.keywords | DNA repeat sequence | |
dc.title | Screening DNA repeat tracts of phase variable genes by fragment analysis | |
dc.type | Book chapter | |
dc.type.description | B1 - Chapters | |
dc.type.code | C - Journal Articles | |
gro.rights.copyright | © 2019 Springer. This is an electronic version of an article published in Methods in Molecular Biology, Vol., 1969:93-104, 2019. Methods in Molecular Biology is available online at: http://link.springer.com/ with the open URL of your article. | |
gro.hasfulltext | Full Text | |
gro.griffith.author | Seib, Kate | |
gro.griffith.author | Jen, Freda E. | |