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dc.contributor.authorSukkaew, Apamas
dc.contributor.authorThanagith, Montri
dc.contributor.authorThongsakulprasert, Tipparat
dc.contributor.authorMutso, Margit
dc.contributor.authorMahalingam, Suresh
dc.contributor.authorSmith, Duncan R
dc.contributor.authorUbol, Sukathida
dc.date.accessioned2019-05-29T12:59:29Z
dc.date.available2019-05-29T12:59:29Z
dc.date.issued2018
dc.identifier.issn0022-1317
dc.identifier.doi10.1099/jgv.0.001039
dc.identifier.urihttp://hdl.handle.net/10072/384164
dc.description.abstractLow-passage clinical isolates of chikungunya virus (CHIKV) were found to be a mixture of large- and small-plaque viruses, with small-plaque viruses being the predominant species. To investigate the contribution of plaque variants to the pathology of the joint, primary human fibroblast-like synoviocytes (HFLS) were used. Large- and small-plaque viruses were purified from two clinical isolates, CHIKV-031C and CHIKV-033C, and were designated CHIKV-031L and CHIKV-031S and CHIKV-033L and CHIKV-033S, respectively. The replication efficiencies of these viruses in HFLSs were compared and it was found that CHIKV-031S and CHIKV-033S replicated with the highest efficiency, while the parental clinical isolates had the lowest efficiency. Interestingly, the cytopathic effects (CPE) induced by these viruses correlated with neither the efficiency of replication nor the plaque size. The small-plaque viruses and the clinical isolates induced cell death rapidly, while large-plaque viruses induced slow CPE in which only 50 % of the cells in infected cultures were rounded up and detached on day 5 of infection. The production of proinflammatory cytokines and chemokines from infected HFLSs was evaluated. The results showed that the large-plaque viruses and the clinical isolates, but not small-plaque variants, were potent inducers of IL-6, IL-8 and MCP-1, and were able to migrate monocytes/macrophages efficiently. Sequencing data revealed a number of differences in amino acid sequences between the small- and large-plaque viruses. The results suggest that it is common for clinical isolates of CHIKV to be heterogeneous, while the variants may have distinct roles in the pathology of the joint.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherMicrobiology Society
dc.relation.ispartofpagefrom525
dc.relation.ispartofpageto535
dc.relation.ispartofissue4
dc.relation.ispartofjournalJournal of General Virology
dc.relation.ispartofvolume99
dc.subject.fieldofresearchBiological sciences
dc.subject.fieldofresearchAgricultural, veterinary and food sciences
dc.subject.fieldofresearchBiomedical and clinical sciences
dc.subject.fieldofresearchMedical microbiology
dc.subject.fieldofresearchcode31
dc.subject.fieldofresearchcode30
dc.subject.fieldofresearchcode32
dc.subject.fieldofresearchcode3207
dc.titleHeterogeneity of clinical isolates of chikungunya virus and its impact on the responses of primary human fibroblast-like synoviocytes
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.hasfulltextNo Full Text
gro.griffith.authorMutso, Margit
gro.griffith.authorMahalingam, Suresh


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