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dc.contributor.authorDay, Christopher J
dc.contributor.authorHartley-Tassell, Lauren E
dc.contributor.authorSeib, Kate L
dc.contributor.authorTiralongo, Joe
dc.contributor.authorBovin, Nicolai
dc.contributor.authorSavino, Silvana
dc.contributor.authorMasignani, Vega
dc.contributor.authorJennings, Michael P
dc.date.accessioned2019-07-02T12:31:14Z
dc.date.available2019-07-02T12:31:14Z
dc.date.issued2019
dc.identifier.issn0006-291X
dc.identifier.doi10.1016/j.bbrc.2019.03.092
dc.identifier.urihttp://hdl.handle.net/10072/384461
dc.description.abstractPseudomonas aeruginosa is an opportunistic pathogen that causes nosocomial infections most commonly in immunocompromised, cystic fibrosis (CF) and burns patients. The pilin and Pseudomonas lectins 1 (PA-IL) and 2 (PA-IIL) are known glycan-binding proteins of P. aeruginosa that are involved in adherence to host cells, particularly CF host airways. Recently, new P. aeruginosa surface proteins were identified by reverse vaccinology and tested in vivo as potential vaccine antigens. Three of these, namely PSE17-1, PSE41-5 and PSE54, were screened for glycan binding using glycan arrays displaying glycan structures representative of those found on human cells. Surface plasmon resonance was used to confirm the lectin activity of these proteins, and determined affinities with several host glycans to be in the nanomolar range. PSE17-1 binds hyaluronic acid and sialyl Lewis A and X. PSE41-5 binds terminal β-linked galactose structures, Lewis and ABO blood group antigens. PSE54 binds to ABO blood group antigens and some terminal β-linked galactose. All three proteins are novel lectins of P. aeruginosa with potential roles in infection of host cells.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherACADEMIC PRESS INC ELSEVIER SCIENCE
dc.relation.ispartofpagefrom287
dc.relation.ispartofpageto290
dc.relation.ispartofissue1
dc.relation.ispartofjournalBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
dc.relation.ispartofvolume513
dc.subject.fieldofresearchBiochemistry and Cell Biology
dc.subject.fieldofresearchMedicinal and Biomolecular Chemistry
dc.subject.fieldofresearchMedical Biochemistry and Metabolomics
dc.subject.fieldofresearchcode0601
dc.subject.fieldofresearchcode0304
dc.subject.fieldofresearchcode1101
dc.titleLectin activity of Pseudomonas aeruginosa vaccine candidates PSE17-1, PSE41-5 and PSE54
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.hasfulltextNo Full Text
gro.griffith.authorSeib, Kate
gro.griffith.authorJennings, Michael P.
gro.griffith.authorDay, Christopher J.
gro.griffith.authorHartley-Tassell, Lauren E.
gro.griffith.authorTiralongo, Joe


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