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  • Cytoprotective effect of atmospheric pressure helium plasma on oxygen and glucose deprivation-induced cell death in H9C2 cardiac myoblasts and primary neonatal rat cardiomyocytes

    Author(s)
    Yan, Xu
    Zhang, Chenyang
    Ouyang, Jiting
    Meng, Zhaozhong
    Shi, Zhongfang
    Wang, Yujiao
    Chen, Ye
    Yuan, Fang
    Ostrikov, Kostya Ken
    Griffith University Author(s)
    Ostrikov, Kostya (Ken)
    Year published
    2019
    Metadata
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    Abstract
    Atmospheric pressure plasma jet (APPJ) has shown excellent potential prospects in biomedical applications, based on the production of reactive oxygen species and reactive nitrogen species (RNS) from APPJ emissions. The current research focused on the protective effect of APPJ on oxygen and glucose deprivation (OGD)-induced cell death in both the H9C2 cardiac myoblast cell line, a frequently used cardiac cell line in cardioprotective studies, and primary neonatal rat cardiomyocytes (NRCMs). Cells were treated with APPJ for different durations, cultured for 6 h and then subjected to OGD for 18 h before their use in assays. We ...
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    Atmospheric pressure plasma jet (APPJ) has shown excellent potential prospects in biomedical applications, based on the production of reactive oxygen species and reactive nitrogen species (RNS) from APPJ emissions. The current research focused on the protective effect of APPJ on oxygen and glucose deprivation (OGD)-induced cell death in both the H9C2 cardiac myoblast cell line, a frequently used cardiac cell line in cardioprotective studies, and primary neonatal rat cardiomyocytes (NRCMs). Cells were treated with APPJ for different durations, cultured for 6 h and then subjected to OGD for 18 h before their use in assays. We found that APPJ treatment could maintain H9C2 cell viability and reduce cell apoptosis in a dose-dependent manner in cells subjected to the OGD conditions. To confirm the cardioprotective effect of APPJ on primary NRCM, we first identified the 'safe dose' of APPJ treatment by evaluating the cytotoxicity of APPJ on primary NRCMs in normal culture conditions. Under the 'safe dose' of APPJ treatment, we also found that the APPJ treatment could maintain NRCM viability under OGD conditions and reduce CK-MB and cTnI release from cardiomyocytes. Further studies revealed that the cytoprotective effect of APPJ may be related to NO production induced by APPJ treatment. Our results gave the first evidence of the cardiotoxicity and cytoprotective effect of APPJ on cardiomyocytes against OGD injury, and furthermore, contributed to new insights into the potential medical applications of plasma in cardiovascular diseases.
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    Journal Title
    JOURNAL OF PHYSICS D-APPLIED PHYSICS
    Volume
    52
    Issue
    13
    DOI
    https://doi.org/10.1088/1361-6463/aafe9a
    Subject
    Physical Sciences
    Engineering
    Publication URI
    http://hdl.handle.net/10072/384737
    Collection
    • Journal articles

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