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dc.contributor.authorChen, S
dc.contributor.authorSandford, S
dc.contributor.authorKirman, JR
dc.contributor.authorRehm, BHA
dc.date.accessioned2019-07-04T12:37:40Z
dc.date.available2019-07-04T12:37:40Z
dc.date.issued2019
dc.identifier.issn0892-6638
dc.identifier.doi10.1096/fj.201802501RR
dc.identifier.urihttp://hdl.handle.net/10072/385157
dc.description.abstractA major obstacle to tuberculosis (TB)-subunit-vaccine development has been the induction of inadequate levels of protective immunity due to the limited breadth of antigen in vaccine preparations. In this study, immunogenic mycobacterial fusion peptides Ag85B-TB10.4 and Ag85B-TB10.4-Rv2660c were covalently displayed on the surface of self-assembled polyester particles. This study investigated whether polyester particles displaying mycobacterial antigens could provide augmented immunogenicity (i.e., offer an innovative vaccine formulation) when compared with free soluble antigens. Herein, polyester particle-based particulate vaccines were produced in an endotoxin-free Escherichia coli strain and emulsified with the adjuvant dimethyl dioctadecyl ammonium bromide. C57BL/6 mice were used to study the immunogenicity of formulated particulate vaccines. The result of humoral immunity showed the antibodies only interacted with target antigens and not with PhaC and the background proteins of the production host. The analysis of T helper 1 cellular immunity indicated that a relatively strong production of cellular immunity biomarkers, IFN-γ and IL-17A cytokines, was induced by particulate vaccines when compared with the respective soluble controls. This study demonstrated that polyester particles have the potential to perform as a mycobacterial antigen-delivery agent to induce augmented antigen-specific immune responses in contrast to free soluble vaccines.-Chen, S., Sandford, S., Kirman, J. R., Rehm, B. H. A. Innovative antigen carrier system for the development of tuberculosis vaccines.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherFASEB
dc.relation.ispartoflocationUnited States
dc.relation.ispartofpagefrom7505
dc.relation.ispartofpageto7518
dc.relation.ispartofissue6
dc.relation.ispartofjournalFASEB journal : official publication of the Federation of American Societies for Experimental Biology
dc.relation.ispartofvolume33
dc.subject.fieldofresearchBiochemistry and cell biology
dc.subject.fieldofresearchZoology
dc.subject.fieldofresearchMedical physiology
dc.subject.fieldofresearchcode3101
dc.subject.fieldofresearchcode3109
dc.subject.fieldofresearchcode3208
dc.titleInnovative antigen carrier system for the development of tuberculosis vaccines
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.hasfulltextNo Full Text
gro.griffith.authorRehm, Bernd
gro.griffith.authorChen, Shuxiong


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