Sub-lethal photocatalysis bactericidal technology cause longer persistence of antibiotic-resistance mutant and plasmid through the mechanism of reduced fitness cost
Author(s)
Yin, Hongliang
Chen, Xiaofang
Li, Guiying
Chen, Yongdi
Wang, Wanjun
An, Taicheng
Wong, Po Keung
Zhao, Huijun
Griffith University Author(s)
Year published
2019
Metadata
Show full item recordAbstract
Antibiotic-resistance bacteria (ARB) sustain longevity in environments representing a public health threat recently, but some current bactericidal technologies could eliminate ARB at endpoint of treatment. However, the intermediate process, where bacterial community might be shaped by a complex array of external stressor, was less to be investigated. Herein, the sub-lethal photocatalysis (Sub-PC) bactericidal technology was first applied to validate its effects on the fitness cost and fate of ARB mediated by mutations and antibiotic-resistance gene (ARG) encoded plasmid. Results revealed that the fitness cost of both two ...
View more >Antibiotic-resistance bacteria (ARB) sustain longevity in environments representing a public health threat recently, but some current bactericidal technologies could eliminate ARB at endpoint of treatment. However, the intermediate process, where bacterial community might be shaped by a complex array of external stressor, was less to be investigated. Herein, the sub-lethal photocatalysis (Sub-PC) bactericidal technology was first applied to validate its effects on the fitness cost and fate of ARB mediated by mutations and antibiotic-resistance gene (ARG) encoded plasmid. Results revealed that the fitness cost of both two types of ARB decreased under Sub-PC conditions as compared with Luria-Broth (LB) broth condition. Plate counting revealed that antibiotic resistance mutant exhibited 6–7 days longer maintenance under the Sub-PC conditions than that under the LB condition. Besides, for plasmid-encoded ARB, the average abundance of ARG in bacteria community was maintained at 0.38 copies for ctx-1 and 0.58 copies for mcr-1 under the Sub-PC condition, which were higher than that under the LB condition (0.29 copies for ctx-1 and 0.45 copies for mcr-1). This study suggests that Sub-PC could cause longer-term maintenance of antibiotic resistance and provides an insightful understanding that how anthropogenic sterilization technologies or stressor may alleviate or spread bacterial antibiotic resistance.
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View more >Antibiotic-resistance bacteria (ARB) sustain longevity in environments representing a public health threat recently, but some current bactericidal technologies could eliminate ARB at endpoint of treatment. However, the intermediate process, where bacterial community might be shaped by a complex array of external stressor, was less to be investigated. Herein, the sub-lethal photocatalysis (Sub-PC) bactericidal technology was first applied to validate its effects on the fitness cost and fate of ARB mediated by mutations and antibiotic-resistance gene (ARG) encoded plasmid. Results revealed that the fitness cost of both two types of ARB decreased under Sub-PC conditions as compared with Luria-Broth (LB) broth condition. Plate counting revealed that antibiotic resistance mutant exhibited 6–7 days longer maintenance under the Sub-PC conditions than that under the LB condition. Besides, for plasmid-encoded ARB, the average abundance of ARG in bacteria community was maintained at 0.38 copies for ctx-1 and 0.58 copies for mcr-1 under the Sub-PC condition, which were higher than that under the LB condition (0.29 copies for ctx-1 and 0.45 copies for mcr-1). This study suggests that Sub-PC could cause longer-term maintenance of antibiotic resistance and provides an insightful understanding that how anthropogenic sterilization technologies or stressor may alleviate or spread bacterial antibiotic resistance.
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Journal Title
APPLIED CATALYSIS B-ENVIRONMENTAL
Volume
245
Subject
Physical chemistry
Chemical engineering
Environmental engineering