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dc.contributor.authorKoralur, Munegowda
dc.contributor.authorSingh, Rahul
dc.contributor.authorVarma, Muralidhar
dc.contributor.authorShenoy, Shalini
dc.contributor.authorAcharya, Vasudeva
dc.contributor.authorKamath, Asha
dc.contributor.authorStenos, John
dc.contributor.authorAthan, Eugen
dc.contributor.authorBairy, Indira
dc.date.accessioned2019-06-10T03:48:01Z
dc.date.available2019-06-10T03:48:01Z
dc.date.issued2018
dc.identifier.issn0732-8893
dc.identifier.doi10.1016/j.diagmicrobio.2018.01.018
dc.identifier.urihttp://hdl.handle.net/10072/385282
dc.description.abstractScrub typhus (ST) is an underdiagnosed acute febrile illness in the Asia Pacific region with recent reemergence. Clinical diagnosis is difficult, and laboratory confirmation is largely based on serological and molecular tests. However, Weil–Felix test still remains the only test available in much of the rural tropics where a disproportionate number of cases occur. Sensitive and affordable assays are important for broader use and accurate diagnosis. We evaluated the diagnostic capabilities of serological and molecular assays on single acute clinical samples. Out of 1036 cases, 319 were confirmed as ST, and the sensitivities of immunofluorescent assay (IFA), IgM enzyme-linked immunosorbent assay (ELISA), nested polymerase chain reaction (n-PCR) and WFT were 93.4%, 80.3%, 75.2%, and 54.2%, respectively. IgM ELISA + n-PCR combination demonstrated highest degree of agreement (κ = .911) in the absence of IFA. Additionally, 16 cases were detected by n-PCR only. Our study emphasizes the diagnostic challenges in the developing world, importance of molecular tests, and best alternate assays in ST diagnosis.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier Science
dc.relation.ispartofpagefrom112
dc.relation.ispartofpageto117
dc.relation.ispartofissue2
dc.relation.ispartofjournalDiagnostic Microbiology and Infectious Disease
dc.relation.ispartofvolume91
dc.subject.fieldofresearchMicrobiology
dc.subject.fieldofresearchClinical sciences
dc.subject.fieldofresearchMedical microbiology
dc.subject.fieldofresearchcode3107
dc.subject.fieldofresearchcode3202
dc.subject.fieldofresearchcode3207
dc.titleScrub typhus diagnosis on acute specimens using serological and molecular assays - a 3-year prospective study
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.hasfulltextNo Full Text
gro.griffith.authorStenos, John


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