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dc.contributor.authorYou, Hong
dc.contributor.authorLiu, Chang
dc.contributor.authorDu, Xiaofeng
dc.contributor.authorNawaratna, Sujeevi
dc.contributor.authorRivera, Vanessa
dc.contributor.authorHarvie, Marina
dc.contributor.authorJones, Malcolm
dc.contributor.authorMcManus, Donald
dc.date.accessioned2019-06-12T12:31:51Z
dc.date.available2019-06-12T12:31:51Z
dc.date.issued2018
dc.identifier.issn1422-0067
dc.identifier.doi10.3390/ijms19082426
dc.identifier.urihttp://hdl.handle.net/10072/385369
dc.description.abstractTo further investigate the importance of Schistosoma japonicum acetylcholinesterase (SjAChE) in cholinergic signaling for parasite growth and development, we used RNA interference (RNAi) to knock-down its expression in adults and eggs in vitro. This resulted in its reduced transcription but also expression of other important genes involved both in cholinergic signaling and glucose uptake were impacted substantially. Significant decreases in AChE protein expression, AChE enzymatic activity, and glucose uptake were observed in the SjAChE-knockdown parasites compared with luciferase controls. In vaccine/challenge experiments, we found that immunization of mice with recombinant SjAChE (rSjAChE) expressed in Escherichia coli elicited reductions in male worm numbers (33%), liver granuloma density (41%), and reduced numbers of mature intestinal eggs (73%) in the vaccinated group compared with the control group. These results indicate AChE plays an important role in the metabolism of male worms, and impacts indirectly on female fecundity leading to increased numbers of immature eggs being released and reduced sizes of liver granulomas. Furthermore, cytokine analysis showed that immunization of mice with rSjAChE elicited a predominantly Th1-type immune response characterized by increased production of IFNγ in splenic CD4+ T cells of vaccinated mice. The study confirms the potential of SjAChE as a vaccine/drug candidate against zoonotic schistosomiasis japonica.</jats:p>
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherMDPI AG
dc.relation.ispartofpagefrom2426
dc.relation.ispartofpageto2426
dc.relation.ispartofissue8
dc.relation.ispartofjournalInternational Journal of Molecular Sciences
dc.relation.ispartofvolume19
dc.subject.fieldofresearchOther chemical sciences
dc.subject.fieldofresearchGenetics
dc.subject.fieldofresearchMicrobiology
dc.subject.fieldofresearchInfectious agents
dc.subject.fieldofresearchOther biological sciences
dc.subject.fieldofresearchcode3499
dc.subject.fieldofresearchcode3105
dc.subject.fieldofresearchcode3107
dc.subject.fieldofresearchcode310702
dc.subject.fieldofresearchcode3199
dc.titleSuppression of Schistosoma japonicum Acetylcholinesterase Affects Parasite Growth and Development
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttp://creativecommons.org/licenses/by/4.0/
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
gro.hasfulltextFull Text
gro.griffith.authorNawaratna, Sujeevi S.


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