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dc.contributor.authorDondossola, Eleonora
dc.contributor.authorAlexander, Stephanie
dc.contributor.authorHolzapfel, Boris M
dc.contributor.authorFilippini, Stefano
dc.contributor.authorStarbuck, Michael W
dc.contributor.authorHoffman, Robert M
dc.contributor.authorNavone, Nora
dc.contributor.authorDe-Juan-Pardo, Elena M
dc.contributor.authorLogothetis, Christopher J
dc.contributor.authorHutmacher, Dietmar W
dc.contributor.authorFriedl, Peter
dc.date.accessioned2019-07-03T12:36:41Z
dc.date.available2019-07-03T12:36:41Z
dc.date.issued2018
dc.identifier.issn1946-6234
dc.identifier.doi10.1126/scitranslmed.aao5726
dc.identifier.urihttp://hdl.handle.net/10072/385716
dc.description.abstractIntravital multiphoton microscopy (iMPM) in mice provides access to cellular and molecular mechanisms of metastatic progression of cancers and the underlying interactions with the tumor stroma. Whereas iMPM of malignant disease has been performed for soft tissues, noninvasive iMPM of solid tumor in the bone is lacking. We combined miniaturized tissue-engineered bone constructs in nude mice with a skin window to noninvasively and repetitively monitor prostate cancer lesions by three-dimensional iMPM. In vivo ossicles developed large central cavities containing mature bone marrow surrounded by a thin cortex and enabled tumor implantation and longitudinal iMPM over weeks. Tumors grew inside the bone cavity and along the cortical bone interface and induced niches of osteoclast activation (focal osteolysis). Interventional bisphosphonate therapy reduced osteoclast kinetics and osteolysis without perturbing tumor growth, indicating dissociation of the tumor-stroma axis. The ossicle window, with its high cavity-to-cortex ratio and long-term functionality, thus allows for the mechanistic dissection of reciprocal epithelial tumor-bone interactions and therapy response.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherAMER ASSOC ADVANCEMENT SCIENCE
dc.relation.ispartofissue452
dc.relation.ispartofjournalSCIENCE TRANSLATIONAL MEDICINE
dc.relation.ispartofvolume10
dc.subject.fieldofresearchBiological sciences
dc.subject.fieldofresearchBiomedical and clinical sciences
dc.subject.fieldofresearchcode31
dc.subject.fieldofresearchcode32
dc.titleIntravital microscopy of osteolytic progression and therapy response of cancer lesions in the bone
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.hasfulltextNo Full Text
gro.griffith.authorHutmacher, Dietmar W.


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