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  • The mutation of Scenedesmus obliquus grown in municipal wastewater by laser combined with ultraviolet

    Author(s)
    Zhou, Xu
    Jin, Wenbiao
    Han, Song-fang
    Li, Xiaoye
    Gao, Shu-Hong
    Chen, Chuan
    Xie, Guo-jun
    Tu, Renjie
    Wang, Qing
    Wang, Qilin
    Griffith University Author(s)
    Wang, Qilin
    Year published
    2019
    Metadata
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    Abstract
    Mutagenetic breeding is an efficient technique for the enhancement of lipid productivity from microalgae. In this study, oil-rich microalga Scenedesmus obliquus were treated by Laser-UV composite mutagenesis. Among the 35 mutant strains, X5 was primely screened. Afterwards, a twice UV mutagenizing was operated on X5, and the optimal mutant strain X5-H13 was obtained. The growth rate, dry weight, lipid yield and lipid content of X5-H13 were 0.698×107 cells/mL·d, 0.99 g/L, 0.49 g/L and 48.8% while cultivated in municipal wastewater, respectively, which were increased by 45%, 58%, 109% and 32% than the original strain. The ...
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    Mutagenetic breeding is an efficient technique for the enhancement of lipid productivity from microalgae. In this study, oil-rich microalga Scenedesmus obliquus were treated by Laser-UV composite mutagenesis. Among the 35 mutant strains, X5 was primely screened. Afterwards, a twice UV mutagenizing was operated on X5, and the optimal mutant strain X5-H13 was obtained. The growth rate, dry weight, lipid yield and lipid content of X5-H13 were 0.698×107 cells/mL·d, 0.99 g/L, 0.49 g/L and 48.8% while cultivated in municipal wastewater, respectively, which were increased by 45%, 58%, 109% and 32% than the original strain. The results of the subculture of repeated mutant showed that the biomass and lipid content of strain X5-H13 were up to 0.99 g/L and 48.8%. The growth of each generation was stable. Furthermore, the random amplified polymorphic DNA analysis indicated that the mutant strain X5-H13 was different from the starting strain, with their genetic similarity coefficient value of 0.815.
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    Journal Title
    Korean Journal of Chemical Engineering
    Volume
    36
    Issue
    6
    DOI
    https://doi.org/10.1007/s11814-019-0273-3
    Subject
    Chemical Engineering
    Mechanical Engineering
    Publication URI
    http://hdl.handle.net/10072/385843
    Collection
    • Journal articles

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