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dc.contributor.authorGorle, Anil K
dc.contributor.authorZhang, Junyong
dc.contributor.authorBerners-Price, Susan J
dc.contributor.authorFarrell, Nicholas P
dc.date.accessioned2020-06-28T23:48:59Z
dc.date.available2020-06-28T23:48:59Z
dc.date.issued2019
dc.identifier.issn1477-9226
dc.identifier.doi10.1039/c9dt00753a
dc.identifier.urihttp://hdl.handle.net/10072/385995
dc.description.abstractReported herein is a detailed NMR and DFT study of the interaction of the 15N-labelled dinuclear platinum anticancer compound [{cis-PtCl(NH3)2}2{μ-H2N(CH2)6NH2}]2+ (15N-1, 1,1/c,c) with 1,2-dihexanoyl-sn-glycero-3-phosphate (DHPA), as a comparison with an earlier study of the interaction of the same water-soluble phospholipid fragment with the geometric trans isomer (1,1/t,t). The reaction of 15N-1 with the sodium salt of DHPA was studied at 298 K, pH ∼ 5.6, by [1H,15N] HSQC 2D NMR spectroscopy. The NMR data, supported by DFT models, provide evidence that the monofunctional DHPA adduct of 15N-1 exists in two conformational forms, with different orientation of the (CH2)6 linker; one has an interaction between the unbound {PtN3Cl} moiety and the coordinated DHPA molecule. Similarly, two bifunctional adduct conformers are identified, in which one has an interaction between the phosphate groups of the two bound DHPA molecules. When compared to the previously reported reactions of 1,1/t,t with DHPA, equilibrium conditions of the 1,1/c,c reaction are reached more slowly (120 h), similar to the reaction with phosphate. The rate constant for the first step of DHPA binding (kL) is slightly lower (1.6 fold) for the cis-compared to the trans-isomer, whereas the rate constant for the reverse reaction is 4-fold lower, resulting in a much greater proportion of DHPA bound species at equilibrium.
dc.description.peerreviewedYes
dc.languageEnglish
dc.publisherRoyal Society of Chemistry
dc.relation.ispartoflocationEngland
dc.relation.ispartofpagefrom9791
dc.relation.ispartofpageto9800
dc.relation.ispartofissue26
dc.relation.ispartofjournalDalton Transactions
dc.relation.ispartofvolume48
dc.relation.urihttp://purl.org/au-research/grants/ARC/DP150100308
dc.relation.grantIDDP150100308
dc.relation.fundersARC
dc.subject.fieldofresearchInorganic Chemistry
dc.subject.fieldofresearchTheoretical and Computational Chemistry
dc.subject.fieldofresearchOther Chemical Sciences
dc.subject.fieldofresearchcode0302
dc.subject.fieldofresearchcode0307
dc.subject.fieldofresearchcode0399
dc.titleInfluence of geometric isomerism on the binding of platinum anticancer agents with phospholipids
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dc.description.versionPost-print
gro.rights.copyright© 2019 Royal Society of Chemistry. This is the author-manuscript version of this paper. Reproduced in accordance with the copyright policy of the publisher. Please refer to the journal website for access to the definitive, published version.
gro.hasfulltextFull Text
gro.griffith.authorBerners-Price, Sue J.
gro.griffith.authorGorle, Anil Kumar
gro.griffith.authorFarrell, Nicholas


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