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dc.contributor.authorLi, Yantao
dc.contributor.authorZhang, Kai
dc.contributor.authorLiu, Porun
dc.contributor.authorChen, Mo
dc.contributor.authorZhong, Yulin
dc.contributor.authorYe, Qingsong
dc.contributor.authorWei, Ming Q
dc.contributor.authorZhao, Huijun
dc.contributor.authorTang, Zhiyong
dc.date.accessioned2019-07-12T04:17:22Z
dc.date.available2019-07-12T04:17:22Z
dc.date.issued2019
dc.identifier.issn0935-9648
dc.identifier.doi10.1002/adma.201901570
dc.identifier.urihttp://hdl.handle.net/10072/386145
dc.description.abstractThe intracellular delivery and functionalization of genetic molecules play critical roles in gene-based theranostics. In particular, the delivery of plasmid DNA (pDNA) with safe nonviral vectors for efficient intracellular gene expression has received increasing attention; however, it still has some limitations. A facile one-pot method is employed to encapsulate pDNA into zeolitic imidazole framework-8 (ZIF-8) and ZIF-8-polymer vectors via biomimetic mineralization and coprecipitation. The pDNA molecules are found to be well distributed inside both nanostructures and benefit from their protection against enzymatic degradation. Moreover, through the use of a polyethyleneimine (PEI) 25 kD capping agent, the nanostructures exhibit enhanced loading capacity, better pH responsive release, and stronger binding affinity to pDNA. From in vitro experiments, the cellular uptake and endosomal escape of the protected pDNA are greatly improved with the superior ZIF-8-PEI 25 kD vector, leading to successful gene expression with high transfection efficacy, comparable to expensive commercial agents. New cost-effective avenues to develop metal-organic-framework-based nonviral vectors for efficient gene delivery and expression are provided.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.relation.ispartoflocationGermany
dc.relation.ispartofpagefrome1901570-1
dc.relation.ispartofpageto1901570-9
dc.relation.ispartofjournalAdvanced Materials
dc.subject.fieldofresearchPhysical Sciences
dc.subject.fieldofresearchChemical Sciences
dc.subject.fieldofresearchEngineering
dc.subject.fieldofresearchcode02
dc.subject.fieldofresearchcode03
dc.subject.fieldofresearchcode09
dc.titleEncapsulation of Plasmid DNA by Nanoscale Metal–Organic Frameworks for Efficient Gene Transportation and Expression
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.rights.copyright© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. This is the peer reviewed version of the following article: Encapsulation of Plasmid DNA by Nanoscale Metal–Organic Frameworks for Efficient Gene Transportation and Expression, Advanced Materials, pp.1901570-1 to 1901570-9, 2019, which has been published in final form at 10.1002/adma.201901570. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving (http://olabout.wiley.com/WileyCDA/Section/id-828039.html)
gro.hasfulltextFull Text
gro.griffith.authorChen, Mo
gro.griffith.authorZhong, Yulin
gro.griffith.authorWei, Ming Q.
gro.griffith.authorZhao, Huijun
gro.griffith.authorTang, Zhiyong
gro.griffith.authorLi, Yantao
gro.griffith.authorZhang, Kai
gro.griffith.authorLiu, Porun


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