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  • Genome- and MS-based mining of antibacterial chlorinated chromones and xanthones from the phytopathogenic fungus Bipolaris sorokiniana strain 11134

    Author(s)
    Han, Jianying
    Zhang, Jingyu
    Song, Zhijun
    Liu, Miaomiao
    Hu, Jiansen
    Hou, Chengjian
    Zhu, Guoliang
    Jiang, Lan
    Xia, Xuekui
    Quinn, Ronald J
    Feng, Yunjiang
    Zhang, Lixin
    Hsiang, Tom
    Liu, Xueting
    Griffith University Author(s)
    Quinn, Ronald J.
    Feng, Yun J.
    Liu, Miaomiao
    Year published
    2019
    Metadata
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    Abstract
    Halogen substituents are important for biological activity in many compounds. Genome-based mining of halogenase along with its biosynthetic gene cluster provided an efficient approach for the discovery of naturally occurring organohalogen compounds. Analysis of the genome sequence of a phytopathogenic fungus Bipolaris sorokiniana 11134 revealed a polyketide gene cluster adjacent to a flavin-dependent halogenase capable of encoding halogenated polyketides, which are rarely reported in phytopathogenic fungi. Furthermore, MS- and UV-guided isolation and purification led to the identification of five chlorine-containing natural ...
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    Halogen substituents are important for biological activity in many compounds. Genome-based mining of halogenase along with its biosynthetic gene cluster provided an efficient approach for the discovery of naturally occurring organohalogen compounds. Analysis of the genome sequence of a phytopathogenic fungus Bipolaris sorokiniana 11134 revealed a polyketide gene cluster adjacent to a flavin-dependent halogenase capable of encoding halogenated polyketides, which are rarely reported in phytopathogenic fungi. Furthermore, MS- and UV-guided isolation and purification led to the identification of five chlorine-containing natural products together with seven other chromones and xanthones. Two of the chlorinated compounds and four chromones are new compounds. Their structures were elucidated by NMR spectroscopic analysis and HRESIMS data. The biosynthetic gene clusters of isolated compounds and their putative biosynthetic pathway are also proposed. One new chlorinated compound showed activity against Staphylococcus aureus, methicillin-resistant S. aureus, and three clinical-resistant S. aureus strains with a shared minimum inhibitory concentration (MIC) of 12.5 μg/mL. Genome-based mining of halogenases combined with high-resolution MS- and UV-guided identification provides an efficient approach to discover new halogenated natural products from microorganisms.
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    Journal Title
    Applied Microbiology and Biotechnology
    Volume
    103
    Issue
    13
    DOI
    https://doi.org/10.1007/s00253-019-09821-z
    Subject
    Environmental sciences
    Publication URI
    http://hdl.handle.net/10072/386214
    Collection
    • Journal articles

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