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dc.contributor.authorBertone, Edoardo
dc.contributor.authorChuang, Ann
dc.contributor.authorBurford, Michele A
dc.contributor.authorHamilton, David P
dc.date.accessioned2019-07-16T04:34:37Z
dc.date.available2019-07-16T04:34:37Z
dc.date.issued2019
dc.identifier.issn1568-9883
dc.identifier.doi10.1016/j.hal.2019.101625
dc.identifier.urihttp://hdl.handle.net/10072/386477
dc.description.abstractIn recent years, in-situ fluorometers have been extensively deployed to monitor cyanobacteria in near real-time. Acceptable accuracy can be achieved between measured pigments and cyanobacteria biovolume provided the cyanobacteria species are known. However, cellular photosynthetic pigment content and measurement interferences are site and species specific and can dramatically affect sensor reliability. We quantified the accuracy of an in-situ fluorometer compared with traditional methods using mono- and mixed cultures of four different cyanobacterial species. We found: (1) lower pigment content in cultures in stationary phase, (2) higher precision with the sensor compared to traditional pigment quantification methods of measuring phycocyanin and chlorophyll a, (3) species-specific relationships between sensor readings and measurements related to biovolume, (4) overestimation of pigments in mixed compared with mono cultures, (5) dissolved organic matter causing a loss in signal proportional to its degree of aromaticity, and (6) potential to quantify the degree of cell lysis with a fluorescent dissolved organic matter sensor. This study has provided important new information on the strengths and limitations of fluorescence sensors. The sensor readings can provide accurate biovolume quantification and species determination for a number of bloom-forming species when sensors are properly compensated and calibrated.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier
dc.relation.ispartofpagefrom101325-1
dc.relation.ispartofpageto101325-14
dc.relation.ispartofjournalHarmful Algae
dc.relation.ispartofvolume87
dc.subject.fieldofresearchEnvironmental Sciences
dc.subject.fieldofresearchBiological Sciences
dc.subject.fieldofresearchcode05
dc.subject.fieldofresearchcode06
dc.titleIn-situ fluorescence monitoring of cyanobacteria: Laboratory-based quantification of species-specific measurement accuracy
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.description.versionAccepted Manuscript (AM)
gro.rights.copyright© 2019 Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International Licence, which permits unrestricted, non-commercial use, distribution and reproduction in any medium, providing that the work is properly cited.
gro.hasfulltextFull Text
gro.griffith.authorBurford, Michele A.
gro.griffith.authorBertone, Edoardo
gro.griffith.authorChuang, Ann W.
gro.griffith.authorHamilton, David P.


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