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  • Recommendations for application of Haemophilus influenzae PCR diagnostics to respiratory specimens for children living in northern Australia: a retrospective re-analysis.

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    Smith-Vaughan245777.pdf (857.1Kb)
    Author(s)
    Beissbarth, J
    Binks, MJ
    Marsh, RL
    Chang, AB
    Leach, AJ
    Smith-Vaughan, HC
    Griffith University Author(s)
    Smith-Vaughan, Heidi
    Year published
    2018
    Metadata
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    Abstract
    Objective: Haemophilus haemolyticus can be misidentified as nontypeable Haemophilus influenzae (NTHi) due to their phenotypic similarities in microbiological culture. This study aimed to determine the prevalence of misidentified NTHi in respiratory specimens from children living in northern Australia. Results: Among respiratory specimens collected in studies between 2010 and 2013, retrospective PCR analysis found that routine culture misidentified H. haemolyticus as NTHi in 0.3% (3/879) of nasal specimens, 25% (14/55) of bronchoalveolar lavage and 40% (12/30) of throat specimens. Therefore, in this population, PCR-based ...
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    Objective: Haemophilus haemolyticus can be misidentified as nontypeable Haemophilus influenzae (NTHi) due to their phenotypic similarities in microbiological culture. This study aimed to determine the prevalence of misidentified NTHi in respiratory specimens from children living in northern Australia. Results: Among respiratory specimens collected in studies between 2010 and 2013, retrospective PCR analysis found that routine culture misidentified H. haemolyticus as NTHi in 0.3% (3/879) of nasal specimens, 25% (14/55) of bronchoalveolar lavage and 40% (12/30) of throat specimens. Therefore, in this population, PCR-based NTHi diagnostics are indicated for throat and bronchoalveolar specimens, but not for nasal specimens.
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    Journal Title
    BMC Research Notes
    Volume
    11
    Issue
    1
    DOI
    https://doi.org/10.1186/s13104-018-3429-z
    Copyright Statement
    © The Author(s) 2018. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
    Subject
    Other Medical and Health Sciences
    Biochemistry and Cell Biology
    Haemophilus haemolyticus (Hh)
    Nasopharynx, bronchoalveolar lavage
    Nontypeable Haemophilus influenzae (NTHi)
    Publication URI
    http://hdl.handle.net/10072/386681
    Collection
    • Journal articles

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