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dc.contributor.authorWigger, Christine
dc.contributor.authorMorris, Peter Stanley
dc.contributor.authorStevens, Matthew
dc.contributor.authorSmith-Vaughan, Heidi
dc.contributor.authorHare, Kim
dc.contributor.authorBeissbarth, Jemima
dc.contributor.authorLeach, Amanda Jane
dc.date.accessioned2019-08-29T01:16:17Z
dc.date.available2019-08-29T01:16:17Z
dc.date.issued2019
dc.identifier.issn0167-7012
dc.identifier.doi10.1016/j.mimet.2018.12.013
dc.identifier.urihttp://hdl.handle.net/10072/386764
dc.description.abstractThis study compared flocked (nylon) swabs and (non-flocked) rayon swabs for the detection of Streptococcus pneumoniae, Haemophilus influenzae and Moraxella catarrhalis in nasopharyngeal samples from 20 enrolled Indigenous children under the age of 6 years living in remote Australian Aboriginal communities, and determined which swab the child or parent perceived to be more comfortable. There was no evidence of a significant difference between flocked and rayon swabs in the recovery of common respiratory bacteria. Rayon swabs detected presence of S. pneumoniae (90% cf. 74%, p = 0.375), H. influenzae (79% cf. 74%, p = 1.00) and M. catarrhalis (79% cf. 74%, p = 1.00) at higher rates than the flocked swabs. Analysis of semi-quantitative growth scores also showed no significant differences in either the ranked distributions or medians. Rayon swabs median semi-quantitative growth scores were higher for S. pneumoniae (4 [IQR 1–5] cf. 3 [IQR 0–6], p = 0.699), and H. influenzae (2 [IQR1–5] cf. 1 [IQR0–5], p = 0.946). Sixty percent of participants preferred samples to be taken with flocked swabs. This study demonstrates that microbiological outcomes are not compromised when using flocked or rayon swabs in respiratory bacterial carriage studies in this population. Therefore, cost, methodological consistency across studies, and participant preference can be considered when choosing swab type.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier
dc.relation.ispartofpagefrom47
dc.relation.ispartofpageto49
dc.relation.ispartofjournalJournal of Microbiological Methods
dc.relation.ispartofvolume157
dc.subject.fieldofresearchMicrobiology
dc.subject.fieldofresearchMedical Microbiology
dc.subject.fieldofresearchcode0605
dc.subject.fieldofresearchcode1108
dc.subject.keywordsScience & Technology
dc.subject.keywordsLife Sciences & Biomedicine
dc.subject.keywordsBiochemical Research Methods
dc.subject.keywordsBiochemistry & Molecular Biology
dc.titleA comparison of flocked nylon swabs and non-flocked rayon swabs for detection of respiratory bacteria in nasopharyngeal carriage in Australian Indigenous children
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationWigger, C; Morris, PS; Stevens, M; Smith-Vaughan, H; Hare, K; Beissbarth, J; Leach, AJ, A comparison of flocked nylon swabs and non-flocked rayon swabs for detection of respiratory bacteria in nasopharyngeal carriage in Australian Indigenous children, Journal of Microbiological Methods, 2019, 157, pp. 47-49
dcterms.dateAccepted2018-12-15
dc.date.updated2019-08-29T01:13:47Z
gro.hasfulltextNo Full Text
gro.griffith.authorSmith-Vaughan, Heidi


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