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dc.contributor.authorRichard, Olivia K
dc.contributor.authorSpringer, Sven
dc.contributor.authorFinzel, Jacqueline
dc.contributor.authorTheuss, Tobias
dc.contributor.authorWyder, Marianne
dc.contributor.authorVidondo, Beatriz
dc.contributor.authorPosthaus, Horst
dc.date.accessioned2019-09-03T00:30:56Z
dc.date.available2019-09-03T00:30:56Z
dc.date.issued2019
dc.identifier.issn2072-6651
dc.identifier.doi10.3390/toxins11040225
dc.identifier.urihttp://hdl.handle.net/10072/386884
dc.description.abstractBackground: Beta-toxin (CPB) is the major virulence factor of Clostridium perfringens type C, causing hemorrhagic enteritis in newborn pigs but also other animals and humans. Vaccines containing inactivated CPB are known to induce protective antibody titers in sow colostrum and neutralization of the CPB activity is thought to be essential for protective immunity in newborn piglets. However, no method is available to quantify the neutralizing effect of vaccine-induced antibody titers in pigs. (2) Methods: We developed a novel assay for the quantification of neutralizing anti-CPB antibodies. Sera and colostrum of sows immunized with a commercial C. perfringens type A and C vaccine was used to determine neutralizing effects on CPB induced cytotoxicity in endothelial cells. Antibody titers of sows and their piglets were determined and compared to results obtained by an ELISA. (3) Results: Vaccinated sows developed neutralizing antibodies against CPB in serum and colostrum. Multiparous sows developed higher serum and colostrum antibody titers after booster vaccinations than uniparous sows. The antibody titers of sows and those of their piglets correlated highly. Piglets from vaccinated sows were protected against intraperitoneal challenge with C. perfringens type C supernatant. (4) Conclusions: The test based on primary porcine endothelial cells quantifies neutralizing antibody activity in serum and colostrum of vaccinated sows and could be used to reduce and refine animal experimentation during vaccine development.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI)
dc.relation.ispartofissue4
dc.relation.ispartofjournalToxins
dc.relation.ispartofvolume11
dc.subject.fieldofresearchBiochemistry and Cell Biology
dc.subject.fieldofresearchPharmacology and Pharmaceutical Sciences
dc.subject.fieldofresearchcode0601
dc.subject.fieldofresearchcode1115
dc.subject.keywordsScience & Technology
dc.subject.keywordsLife Sciences & Biomedicine
dc.subject.keywordsFood Science & Technology
dc.subject.keywordsToxicology
dc.subject.keywordsnecrotizing enteritis
dc.titleApplication of an Endothelial Cell Culture Assay for the Detection of Neutralizing Anti-Clostridium Perfringens Beta-Toxin Antibodies in a Porcine Vaccination Trial
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationRichard, OK; Springer, S; Finzel, J; Theuss, T; Wyder, M; Vidondo, B; Posthaus, H, Application of an Endothelial Cell Culture Assay for the Detection of Neutralizing Anti-Clostridium Perfringens Beta-Toxin Antibodies in a Porcine Vaccination Trial, Toxins, 2019, 11 (4)
dcterms.dateAccepted2019-04-09
dcterms.licensehttps://creativecommons.org/licenses/by/4.0/
dc.date.updated2019-09-03T00:27:05Z
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
gro.hasfulltextFull Text
gro.griffith.authorWyder, Marianne


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