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dc.contributor.authorGao, Qi
dc.contributor.authorXie, Jiajun
dc.contributor.authorSalero, Enrique
dc.contributor.authorSabater, Alfonso L
dc.contributor.authorGaidosh, Gabriel
dc.contributor.authorDe Juan-Pardo, Elena
dc.contributor.authorHutmacher, Dietmar W
dc.contributor.authorYe, Juan
dc.contributor.authorPerez, Victor L
dc.date.accessioned2019-09-05T05:25:55Z
dc.date.available2019-09-05T05:25:55Z
dc.date.issued2017
dc.identifier.issn0146-0404
dc.identifier.urihttp://hdl.handle.net/10072/386959
dc.description.abstractPurpose : Regeneration of corneal stroma is believed to be among the greatest challenges in corneal tissue engineering. The aim of this study is to investigate the effect of a 3D-printed nano-structural poly(ε-caprolactone) (PCL) scaffold on the material-cellular interaction, and to evaluate the efficiency for the corneal stroma regeneration in vitro. Methods : Two nano-structural scaffolds of different design and fiber distribution were fabricated by melt electrospinning 3D printing method using PCL. Human keratocytes were isolated from collagenase-digested limbal stromal tissue and seeded on the 3D-printed PCL scaffold. Cell morphology on scaffolds was studied by confocal microscopy, and cell proliferation was analyzed by PrestoBlue assay. Extracellular matrix(ECM) produced by keratocytes on the scaffolds was characterized by scanning electron microscope(SEM) and two-photon fluorescent microscopy. Results : The 3D-printed PCL scaffolds exhibited different micro-structure under SEM. As revealed in Figure.1, scaffold on the left presented offset fiber distribution, while the other one showed a fiber distribution at regular intervals. SEM and two-photon fluorescent microscopy demonstrated that keratocytes tended to grow along the fibrous network, and spread well on both scaffolds. Collagenous ECM produced by cells on the regular-fibrous scaffold was more abundant than on the offset-fibrous scaffold, forming a relatively thicker cross-section (Figure 2). Compared to a collagen fibrillar network secreted on regular-fibrous scaffolds, a lamellar ultrastructural ECM composed of aligned collagen was remodeled on offset-fibrous scaffolds, which was similar to native cornea stroma. Immunofluorescence staining showed that regular-fibrous scaffolds induced less secretion of type I collagen (COLI) and type V collagen (COLV) than offset-fibrous scaffolds. Conclusions : Our study showed that the fiber composition and topography of 3D-printed nanofibrous PCL scaffolds have a significant effect on the cell behavior through the material-cellular interaction. The refined design of nano-structure by melt electrospinning method stimulates self-organization and self-adaption of ECM, which helps mimicking native tissue microenvironments and offering the potential as cornea stroma regeneration substitutes.
dc.languageEnglish
dc.publisherAssociation for Research in Vision and Ophthalmology (ARVO)
dc.publisher.urihttps://iovs.arvojournals.org/article.aspx?articleid=2638311
dc.relation.ispartofconferencenameAnnual Meeting of the Association for Research in Vision and Ophthalmology (ARVO)
dc.relation.ispartofconferencetitleInvestigative Ophthalmology & Visual Science (IOVS)
dc.relation.ispartofdatefrom2017-05-07
dc.relation.ispartofdateto2017-05-11
dc.relation.ispartoflocationBaltimore, MD
dc.relation.ispartofissue8
dc.relation.ispartofvolume58
dc.subject.fieldofresearchBiological sciences
dc.subject.fieldofresearchBiomedical and clinical sciences
dc.subject.fieldofresearchcode31
dc.subject.fieldofresearchcode32
dc.subject.keywordsScience & Technology
dc.subject.keywordsLife Sciences & Biomedicine
dc.subject.keywordsOphthalmology
dc.title3D-printed Nano-structural Poly(ε-caprolactone) (PCL) Scaffolds for Corneal Stromal Regeneration
dc.typeConference output
dc.type.descriptionE3 - Conferences (Extract Paper)
dcterms.bibliographicCitationGao, Q; Xie, J; Salero, E; Sabater, AL; Gaidosh, G; De Juan-Pardo, E; Hutmacher, DW; Ye, J; Perez, VL, 3D-printed Nano-structural Poly(ε-caprolactone) (PCL) Scaffolds for Corneal Stromal Regeneration, Investigative Ophthalmology & Visual Science (IOVS), 2017, 58 (8)
dc.date.updated2019-09-05T05:21:29Z
gro.hasfulltextNo Full Text
gro.griffith.authorHutmacher, Dietmar W.


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