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dc.contributor.authorWu, Yanheng
dc.contributor.authorGu, Wenyi
dc.contributor.authorChen, Chen
dc.contributor.authorSon, Trong Do
dc.contributor.authorXu, Zhi Ping
dc.date.accessioned2019-09-10T02:52:14Z
dc.date.available2019-09-10T02:52:14Z
dc.date.issued2018
dc.identifier.issn2470-1343
dc.identifier.doi10.1021/acsomega.8b00397
dc.identifier.urihttp://hdl.handle.net/10072/387159
dc.description.abstractLayered double hydroxide (LDH) nanoparticles (NPs) are safe and effective vectors for small interfering RNA (siRNA) delivery. However, it is unclear whether there are optimal parameters for the efficient delivery of functional siRNA using LDH NPs. In this research, we comprehensively examined the effect of parameters, such as the mixing method and LDH/siRNA mass ratio on siRNA silencing capability. We first noted that the best way for loading gene segments (25 bp dsDNA and siRNA) is to add gene molecules to 100 nm LDH and then diluting in Dulbecco's modified Eagle's medium. Very interestingly, the optimal LDH/gene mass ratio is around 20:1 in terms of cellular uptake amount of gene segments, whereas this ratio is shifted to around 5:1 in terms of target gene silencing efficacy, which has been reasonably explained. The optimization for LDH NP-based gene delivery system may provide the guidance for more efficient in vitro and in vivo siRNA delivery using the optimal parameters.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherAmerican Chemical Society (ACS Publications)
dc.relation.ispartofpagefrom4871
dc.relation.ispartofpageto4877
dc.relation.ispartofissue5
dc.relation.ispartofjournalACS Omega
dc.relation.ispartofvolume3
dc.subject.fieldofresearchChemical Engineering
dc.subject.fieldofresearchMaterials Engineering
dc.subject.fieldofresearchcode0904
dc.subject.fieldofresearchcode0912
dc.subject.keywordsScience & Technology
dc.subject.keywordsPhysical Sciences
dc.subject.keywordsChemistry, Multidisciplinary
dc.subject.keywordsUPTAKE MECHANISM
dc.titleOptimization of Formulations Consisting of Layered Double Hydroxide Nanoparticles and Small Interfering RNA for Efficient Knockdown of the Target Gene
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationWu, Y; Gu, W; Chen, C; Son, TD; Xu, ZP, Optimization of Formulations Consisting of Layered Double Hydroxide Nanoparticles and Small Interfering RNA for Efficient Knockdown of the Target Gene, ACS Omega, 2018, 3 (5), pp. 4871-4877
dcterms.dateAccepted2018-04-12
dc.date.updated2019-09-10T02:48:17Z
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© 2018 American Chemical Society. This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
gro.hasfulltextFull Text
gro.griffith.authorChen, Chen


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