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dc.contributor.authorShi, Ting-Ting
dc.contributor.authorYang, Fang-Yuan
dc.contributor.authorLiu, Chang
dc.contributor.authorCao, Xi
dc.contributor.authorLu, Jing
dc.contributor.authorZhang, Xue-Lian
dc.contributor.authorYuan, Ming-Xia
dc.contributor.authorChen, Chen
dc.contributor.authorYang, Jin-Kui
dc.date.accessioned2019-09-10T03:42:03Z
dc.date.available2019-09-10T03:42:03Z
dc.date.issued2018
dc.identifier.issn0006-291X
dc.identifier.doi10.1016/j.bbrc.2017.11.055
dc.identifier.urihttp://hdl.handle.net/10072/387173
dc.description.abstractMitochondrial metabolism plays an essential role in the regulation of insulin release and glucose homeostasis. Evidence demonstrated that the angiotensin-converting enzyme 2 (ACE2) participates in the regulation of glucose metabolism, however, its role in mitochondrial metabolism remains unclear. The purpose of our study was to determine if ACE2 can regulate mitochondrial function in pancreatic β-cells. We found that ACE2 over-expression restored glucose-stimulated insulin secretion (GSIS) and mitochondrial membrane potential (MMP) in the presence of H 2 O 2 in INS-1 cells. PCR array demonstrated that ACE2 over-expression up-regulated 67 mitochondria-related genes in INS-1 cells. In pancreatic islets, ACE2 ablation attenuated intracellular calcium influx with a decrease in GSIS. Ace2 −/y mice islets exhibited impaired mitochondrial respiration and lower production of ATP, along with decreased expression of genes involved in mitochondrial oxidation. In islets from db/db mice, ACE2 over-expression increased intracellular calcium influx and restored impaired mitochondrial oxidation, potentially causing an increase in GSIS. These results shed light on the potential roles of ACE2 in mitochondrial metabolism, moreover, may improve our understanding of diabetes.
dc.description.peerreviewedYes
dc.languageEnglish
dc.publisherElsevier
dc.relation.ispartofpagefrom860
dc.relation.ispartofpageto866
dc.relation.ispartofissue1
dc.relation.ispartofjournalBiochemical and Biophysical Research Communications
dc.relation.ispartofvolume495
dc.subject.fieldofresearchMedicinal and Biomolecular Chemistry
dc.subject.fieldofresearchBiochemistry and Cell Biology
dc.subject.fieldofresearchMedical Biochemistry and Metabolomics
dc.subject.fieldofresearchcode0304
dc.subject.fieldofresearchcode0601
dc.subject.fieldofresearchcode1101
dc.subject.keywordsScience & Technology
dc.subject.keywordsLife Sciences & Biomedicine
dc.subject.keywordsBiochemistry & Molecular Biology
dc.subject.keywordsBiophysics
dc.subject.keywordsACE2
dc.titleAngiotensin-converting enzyme 2 regulates mitochondrial function in pancreatic beta-cells
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationShi, T-T; Yang, F-Y; Liu, C; Cao, X; Lu, J; Zhang, X-L; Yuan, M-X; Chen, C; Yang, J-K, Angiotensin-converting enzyme 2 regulates mitochondrial function in pancreatic beta-cells, Biochemical and Biophysical Research Communications, 2018, 495 (1), pp. 860-866
dcterms.dateAccepted2017-11-08
dc.date.updated2019-09-10T03:40:37Z
gro.hasfulltextNo Full Text
gro.griffith.authorChen, Chen


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