Show simple item record

dc.contributor.authorMojica, Sergio A
dc.contributor.authorEriksson, Anna U
dc.contributor.authorDavis, Rohan A
dc.contributor.authorBahnan, Weel
dc.contributor.authorElofsson, Mikael
dc.contributor.authorGylfe, Asa
dc.date.accessioned2019-09-13T05:13:49Z
dc.date.available2019-09-13T05:13:49Z
dc.date.issued2018
dc.identifier.issn1664-302X
dc.identifier.doi10.3389/fmicb.2018.03151
dc.identifier.urihttp://hdl.handle.net/10072/387307
dc.description.abstractIn this study, we describe the application of a transformed Chlamydia trachomatis strain constitutively expressing the red fluorescent protein mCherry, to allow real-time monitoring of the infection cycle and screening for agents that block replication of C. trachomatis. The red fluorescent C. trachomatis strain was detected autonomously without antibody staining and was equally susceptible to doxycycline as the wild type strain. A high-throughput screening assay was developed using the transformed strain and automated fluorescence microscopy. The assay was used in a pilot screen of a 349 compound library containing natural products from Australian flora and fauna. Compounds with anti-chlamydial activity were tested for dose response and toxicity to host cells and two non-toxic compounds had 50% effective concentration (EC50) values in the low micromolar range. Natural products are valuable sources for drug discovery and the identified Chlamydia growth inhibition may be starting points for future drug development. Live cell imaging was used to visualize growth of the red fluorescent C. trachomatis strain over time. The screening assay reduced workload and reagents compared to an assay requiring immunostaining and could further be used to monitor the development of Chlamydia inclusions and anti-chlamydial effect in real time.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherFrontiers Media
dc.relation.ispartofpagefrom3151:1
dc.relation.ispartofpageto3151:13
dc.relation.ispartofjournalFrontiers in Microbiology
dc.relation.ispartofvolume9
dc.subject.fieldofresearchMicrobiology
dc.subject.fieldofresearchSoil sciences
dc.subject.fieldofresearchcode3107
dc.subject.fieldofresearchcode4106
dc.subject.keywordsScience & Technology
dc.subject.keywordsLife Sciences & Biomedicine
dc.subject.keywordsChlamydia trachomatis
dc.subject.keywordshigh content screening
dc.titleRed Fluorescent Chlamydia trachomatis Applied to Live Cell Imaging and Screening for Antibacterial Agents
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationMojica, SA; Eriksson, AU; Davis, RA; Bahnan, W; Elofsson, M; Gylfe, A, Red Fluorescent Chlamydia trachomatis Applied to Live Cell Imaging and Screening for Antibacterial Agents, Frontiers in Microbiology, 2018, 9, pp.3151:1 - 3151:13
dcterms.dateAccepted2018-12-05
dcterms.licensehttp://creativecommons.org/licenses/by/4.0/
dc.date.updated2019-09-13T05:10:36Z
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© 2018 Mojica, Eriksson, Davis, Bahnan, Elofsson and Gylfe. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
gro.hasfulltextFull Text
gro.griffith.authorDavis, Rohan A.


Files in this item

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record