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dc.contributor.authorTan, Aimee
dc.contributor.authorLi, Wing-Sze
dc.contributor.authorVerderosa, Anthony D
dc.contributor.authorBlakeway, Luke
dc.contributor.authorMubaiwa, Tsitsi D
dc.contributor.authorTotsika, Makrina
dc.contributor.authorSeib, Kate L
dc.date.accessioned2019-09-17T01:03:11Z
dc.date.available2019-09-17T01:03:11Z
dc.date.issued2019
dc.identifier.issn2045-2322
dc.identifier.doi10.1038/s41598-019-39374-0
dc.identifier.urihttp://hdl.handle.net/10072/387388
dc.description.abstractMoraxella catarrhalis is a host-adapted bacterial pathogen that causes otitis media and exacerbations of chronic obstructive pulmonary disease. This study characterises the conserved M. catarrhalis extracellular nuclease, a member of the ββα metal finger family of nucleases, that we have named NucM. NucM shares conserved sequence motifs from the ββα nuclease family, including the DRGH catalytic core and Mg2+ co-ordination site, but otherwise shares little primary sequence identity with other family members, such as the Serratia Nuc and pneumococcal EndA nucleases. NucM is secreted from the cell and digests linear and circular nucleic acid. However, it appears that a proportion of NucM is also associated with the cell membrane and acts as an entry nuclease, facilitating transformation of M. catarrhalis cells. This is the first example of a ββα nuclease in a Gram negative bacteria that acts as an entry nuclease. In addition to its role in competence, NucM affects cell aggregation and biofilm formation by M. catarrhalis, with ΔnucM mutants having increased biofilm biomass. NucM is likely to increase the ability of cells to survive and persist in vivo, increasing the virulence of M. catarrhalis and potentially affecting the behaviour of other pathogens that co-colonise the otorhinolaryngological niche.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherNature Publishing Group
dc.relation.ispartofissue1
dc.relation.ispartofjournalScientific Reports
dc.relation.ispartofvolume9
dc.relation.urihttp://purl.org/au-research/grants/NHMRC/APP1099279
dc.relation.urihttp://purl.org/au-research/grants/NHMRC/APP1045235
dc.relation.grantIDAPP1099279
dc.relation.grantIDAPP1045235
dc.relation.fundersNHMRC
dc.subject.fieldofresearchBiochemistry and Cell Biology
dc.subject.fieldofresearchOther Physical Sciences
dc.subject.fieldofresearchcode0601
dc.subject.fieldofresearchcode0299
dc.subject.keywordsScience & Technology
dc.subject.keywordsMultidisciplinary Sciences
dc.subject.keywordsScience & Technology - Other Topics
dc.subject.keywordsSTREPTOCOCCUS-PNEUMONIAE
dc.subject.keywordsHAEMOPHILUS-INFLUENZAE
dc.titleMoraxella catarrhalis NucM is an entry nuclease involved in extracellular DNA and RNA degradation, cell competence and biofilm scaffolding
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationTan, A; Li, W-S; Verderosa, AD; Blakeway, L; Mubaiwa, TD; Totsika, M; Seib, KL, Moraxella catarrhalis NucM is an entry nuclease involved in extracellular DNA and RNA degradation, cell competence and biofilm scaffolding, Scientific Reports, 2019, 9 (1)
dcterms.dateAccepted2019-01-18
dcterms.licensehttp://creativecommons.org/licenses/by/4.0/
dc.date.updated2019-09-17T01:00:51Z
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© The Author(s) 2019. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
gro.hasfulltextFull Text
gro.griffith.authorSeib, Kate
gro.griffith.authorTan, Aimee
gro.griffith.authorMubaiwa, Tsitsi D.
gro.griffith.authorBlakeway, Luke V.


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