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  • Microfluidic-Based Nucleic Acid Amplification Systems in Microbiology

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    Gorgannezhad232794Published.pdf (881.9Kb)
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    Author(s)
    Gorgannezhad, Lena
    Stratton, Helen
    Nam-Trung, Nguyen
    Griffith University Author(s)
    Stratton, Helen M.
    Nguyen, Nam-Trung
    Year published
    2019
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    Abstract
    Rapid, sensitive, and selective bacterial detection is a hot topic, because the progress in this research area has had a broad range of applications. Novel and innovative strategies for detection and identification of bacterial nucleic acids are important for practical applications. Microfluidics is an emerging technology that only requires small amounts of liquid samples. Microfluidic devices allow for rapid advances in microbiology, enabling access to methods of amplifying nucleic acid molecules and overcoming difficulties faced by conventional. In this review, we summarize the recent progress in microfluidics-based ...
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    Rapid, sensitive, and selective bacterial detection is a hot topic, because the progress in this research area has had a broad range of applications. Novel and innovative strategies for detection and identification of bacterial nucleic acids are important for practical applications. Microfluidics is an emerging technology that only requires small amounts of liquid samples. Microfluidic devices allow for rapid advances in microbiology, enabling access to methods of amplifying nucleic acid molecules and overcoming difficulties faced by conventional. In this review, we summarize the recent progress in microfluidics-based polymerase chain reaction devices for the detection of nucleic acid biomarkers. The paper also discusses the recent development of isothermal nucleic acid amplification and droplet-based microfluidics devices. We discuss recent microfluidic techniques for sample preparation prior to the amplification process.
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    Journal Title
    Micromachines
    Volume
    10
    Issue
    6
    DOI
    https://doi.org/10.3390/mi10060408
    Copyright Statement
    © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
    Subject
    Nanotechnology
    Science & Technology
    Technology
    Nanoscience & Nanotechnology
    Instruments & Instrumentation
    Science & Technology - Other Topics
    Publication URI
    http://hdl.handle.net/10072/387507
    Collection
    • Journal articles

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