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dc.contributor.authorOhya, Susumu
dc.contributor.authorIto, Katsunori
dc.contributor.authorHatano, Noriyuki
dc.contributor.authorOhno, Akitoshi
dc.contributor.authorMuraki, Katsuhiko
dc.contributor.authorImaizumi, Yuji
dc.date.accessioned2019-09-25T05:11:49Z
dc.date.available2019-09-25T05:11:49Z
dc.date.issued2019
dc.identifier.issn1661-6596
dc.identifier.doi10.3390/ijms20174073
dc.identifier.urihttp://hdl.handle.net/10072/387741
dc.description.abstractA-type K+ channels contribute to regulating the propagation and frequency of action potentials in smooth muscle cells (SMCs). The present study (i) identified the molecular components of A-type K+ channels in rat vas deferens SMs (VDSMs) and (ii) showed the long-term, genomic effects of testosterone on their expression in VDSMs. Transcripts of the A-type K+ channel α subunit, Kv4.3L and its regulatory β subunits, KChIP3, NCS1, and DPP6-S were predominantly expressed in rat VDSMs over the other related subtypes (Kv4.2, KChIP1, KChIP2, KChIP4, and DPP10). A-type K+ current (IA) density in VDSM cells (VDSMCs) was decreased by castration without changes in IA kinetics, and decreased IA density was compensated for by an oral treatment with 17α-methyltestosterone (MET). Correspondingly, in the VDSMs of castrated rats, Kv4.3L and KChIP3 were down-regulated at both the transcript and protein expression levels. Changes in Kv4.3L and KChIP3 expression levels were compensated for by the treatment with MET. These results suggest that testosterone level changes in testosterone disorders and growth processes control the functional expression of A-type K+ channels in VDSMCs.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherMDPI AG
dc.publisher.placeSwitzerland
dc.relation.ispartofpagefrom4073: 1
dc.relation.ispartofpageto4073: 14
dc.relation.ispartofissue17
dc.relation.ispartofjournalInternational Journal of Molecular Sciences
dc.relation.ispartofvolume20
dc.subject.fieldofresearchClinical Sciences
dc.subject.fieldofresearchOther Chemical Sciences
dc.subject.fieldofresearchGenetics
dc.subject.fieldofresearchOther Biological Sciences
dc.subject.fieldofresearchcode1103
dc.subject.fieldofresearchcode0399
dc.subject.fieldofresearchcode0604
dc.subject.fieldofresearchcode0699
dc.subject.keywordsA-type K+ channel
dc.subject.keywordsKV4.3
dc.subject.keywordscastration
dc.subject.keywordssmooth muscle
dc.subject.keywordstestosterone
dc.titleCastration Induces Down-Regulation of A-Type K+ Channel in Rat Vas Deferens Smooth Muscle.
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationOhya, S; Ito, K; Hatano, N; Ohno, A; Muraki, K; Imaizumi, Y, Castration Induces Down-Regulation of A-Type K+ Channel in Rat Vas Deferens Smooth Muscle., International Journal of Molecular Sciences, 2019, 20 (17), pp. 4073: 1-4073: 14
dcterms.dateAccepted2019-08-19
dcterms.licensehttp://creativecommons.org/licenses/by/4.0/
dc.date.updated2019-09-25T03:21:55Z
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
gro.hasfulltextFull Text
gro.griffith.authorMuraki, Katsuhiko


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