Show simple item record

dc.contributor.authorEbeyan, Samantha
dc.contributor.authorWindsor, Madeline
dc.contributor.authorBordin, Amanda
dc.contributor.authorMhango, Lebogang
dc.contributor.authorErskine, Simon
dc.contributor.authorTrembizki, Ella
dc.contributor.authorMokany, Elisa
dc.contributor.authorTan, Lit Yeen
dc.contributor.authorWhiley, David
dc.contributor.authorGuy, Rebecca
dc.contributor.authorGuy, Rebecca
dc.contributor.authorWand, Handan
dc.contributor.authorChen, Marcus
dc.contributor.authorNimmo, Graeme
dc.contributor.authoret al.
dc.date.accessioned2019-09-27T01:15:51Z
dc.date.available2019-09-27T01:15:51Z
dc.date.issued2019
dc.identifier.issn0305-7453
dc.identifier.doi10.1093/jac/dkz108
dc.identifier.urihttp://hdl.handle.net/10072/387826
dc.description.abstractObjectives: To evaluate the performance of the ResistancePlus GC (beta) assay for the simultaneous detection of Neisseria gonorrhoeae and gyrA S91 markers of resistance (S91F) and susceptibility (WT) to ciprofloxacin, from both clinical specimens and isolates. Methods: Performance was assessed on several sample banks, including N. gonorrhoeae isolates (n = 822), non-gonococcal isolates (n = 110), N. gonorrhoeae-positive clinical specimens (n = 402) and N. gonorrhoeae-negative specimens (n = 290). Results were compared with previous testing data, including S91 genotyping and phenotypic resistance profiles. Results: Overall, the assay demonstrated 100% sensitivity for N. gonorrhoeae detection in clinical isolates. For gyrA S91 mutation detection in clinical isolates, the assay showed 100% sensitivity/specificity compared with the genotype, and >99%/>97% sensitivity/specificity when compared with phenotype. For positive clinical specimens, the assay demonstrated >96% sensitivity for N. gonorrhoeae detection and 100% sensitivity/specificity for gyrA S91 mutation detection. The assay demonstrated >99% specificity for N. gonorrhoeae detection against non-gonococcal isolates and 100% specificity for negative clinical specimens. Conclusions: The ResistancePlus GC (beta) assay is suitable for the detection of N. gonorrhoeae and gyrA markers associated with resistance/susceptibility to ciprofloxacin directly in clinical samples. This assay could be implemented for the individualized treatment of gonorrhoea infections as well as to enhance current antimicrobial resistance surveillance methods.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherOxford University Press
dc.relation.ispartofpagefrom1820
dc.relation.ispartofpageto1824
dc.relation.ispartofissue7
dc.relation.ispartofjournalJournal of Antimicrobial Chemotherapy
dc.relation.ispartofvolume74
dc.subject.fieldofresearchMicrobiology
dc.subject.fieldofresearchMedical Microbiology
dc.subject.fieldofresearchPharmacology and Pharmaceutical Sciences
dc.subject.fieldofresearchcode0605
dc.subject.fieldofresearchcode1108
dc.subject.fieldofresearchcode1115
dc.subject.keywordsScience & Technology
dc.subject.keywordsLife Sciences & Biomedicine
dc.subject.keywordsInfectious Diseases
dc.subject.keywordsPharmacology & Pharmacy
dc.titleEvaluation of the ResistancePlus GC (beta) assay: a commercial diagnostic test for the direct detection of ciprofloxacin susceptibility or resistance in Neisseria gonorrhoeae
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationEbeyan et al., Evaluation of the ResistancePlus GC (beta) assay: a commercial diagnostic test for the direct detection of ciprofloxacin susceptibility or resistance in Neisseria gonorrhoeae, Journal of Antimicrobial Chemotherapy, 2019, 74 (7), pp. 1820-1824
dcterms.dateAccepted2019-02-22
dc.date.updated2019-09-27T01:12:15Z
gro.hasfulltextNo Full Text
gro.griffith.authorNimmo, Graeme R.


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record