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dc.contributor.authorMaroof, Hamidreza
dc.contributor.authorIslam, Farhadul
dc.contributor.authorDong, LanFeng
dc.contributor.authorAjjikuttira, Prabha
dc.contributor.authorGopalan, Vinod
dc.contributor.authorMcMillan, Nigel AJ
dc.contributor.authorLam, Alfred K
dc.date.accessioned2019-09-30T01:15:57Z
dc.date.available2019-09-30T01:15:57Z
dc.date.issued2018
dc.identifier.issn2073-4409
dc.identifier.doi10.3390/cells7120265
dc.identifier.urihttp://hdl.handle.net/10072/387890
dc.description.abstractThis study aims to determine the functional roles of microRNA-34b-5p (miR-34b) in the suppression of anaplastic thyroid carcinoma. We used hydration-of-freeze-dried-matrix (HFDM) formulated liposomes (liposome-loaded miR-34b) for effective delivery of miR-34b to anaplastic thyroid carcinoma in vitro and in vivo. Real time polymerase chain was used to determine the level of miR-34b. Immunocytochemistry, Western blot and ELISA were carried out to determine the effect of this manipulation on VEGF-A expression. In addition, an in vivo xenotransplantation mouse model was used to investigate the functional roles of overexpression of miR-34b in the carcinoma. In anaplastic thyroid carcinoma cells, miR-34b expression was low and significant overexpression (p < 0.05) was noted following transfection with liposome-loaded miR-34b. The miR-34b overexpressed thyroid carcinoma cell lines showed reduction in VEGF-A protein expression, decreased cell proliferation, decreased wound healing, reduced cell cycle progression and increased apoptosis (p < 0.05). In in vivo experiments, when compared to control groups, smaller tumours formed upon intravenous administration of liposome-loaded miR-34b. To conclude, the current study confirmed the tumour suppressor properties of miR-34b via VEGF-A regulation in anaplastic thyroid carcinoma. In addition, delivery of miR-34b using cationic liposome could be a useful therapeutic strategy for targeting therapy in the carcinoma.
dc.description.peerreviewedYes
dc.languageEnglish
dc.publisherMDPI
dc.relation.ispartofpagefrom265:1
dc.relation.ispartofpageto265:16
dc.relation.ispartofissue12
dc.relation.ispartofjournalCells
dc.relation.ispartofvolume7
dc.subject.keywordsScience & Technology
dc.subject.keywordsLife Sciences & Biomedicine
dc.subject.keywordsCell Biology
dc.subject.keywordsmiR-34b
dc.subject.keywordsanaplastic thyroid carcinoma
dc.titleLiposomal Delivery of miR-34b-5p Induced Cancer Cell Death in Thyroid Carcinoma
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationMaroof, H; Islam, F; Dong, L; Ajjikuttira, P; Gopalan, V; McMillan, NAJ; Lam, AK, Liposomal Delivery of miR-34b-5p Induced Cancer Cell Death in Thyroid Carcinoma, Cells, 2018, 7 (12), pp. 265:1-265:16
dcterms.dateAccepted2018-12-10
dcterms.licensehttp://creativecommons.org/licenses/by/4.0/
dc.date.updated2019-09-30T01:07:59Z
dc.description.versionPublished
gro.rights.copyright© 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
gro.hasfulltextFull Text
gro.griffith.authorGopalan, Vinod
gro.griffith.authorDong, Lan-feng
gro.griffith.authorMcMillan, Nigel
gro.griffith.authorLam, Alfred K.
gro.griffith.authorIslam, Mohammed F.
gro.griffith.authorMaroof, Hamidreza


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